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BUB3 (BUB3 mitotic checkpoint protein)

Written2019-03Jorge Antonio Elias Godoy Carlos, João Agostinho Machado-Neto
Department of Pharmacology, Institute of Biomedical Sciences of the University of São Paulo, São Paulo, Brazil;;

Abstract BUB3 is a WD40 protein that belongs to spindle mitotic checkpoint complex, which monitors the chromosome attachment to mitotic (or meiotic) fuse and prevents premature chromosome segregation. Alterations in BUB3 have been associated with chromosomal instability and aneuploidy, but their contribution for cancer development and progression are poorly understood, and appear to differ depending on the type of cancer. The present review contains data on BUB3 DNA, RNA, protein encoded and function.

Keywords BUB3; Mitotic checkpoint protein BUB3; WD40 protein; Spindle checkpoint; Cell cycle; Anaphase-promoting complex.

(Note : for Links provided by Atlas : click)


HGNC (Hugo) BUB3
HGNC Alias symbBUB3L
HGNC Previous nameBUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog
 budding uninhibited by benzimidazoles 3 homolog (yeast)
LocusID (NCBI) 9184
Atlas_Id 855
Location 10q26.13  [Link to chromosome band 10q26]
Location_base_pair Starts at 123154402 and ends at 123170467 bp from pter ( according to GRCh38/hg38-Dec_2013)  [Mapping BUB3.png]
Fusion genes
(updated 2017)
Data from Atlas, Mitelman, Cosmic Fusion, Fusion Cancer, TCGA fusion databases with official HUGO symbols (see references in chromosomal bands)


Description The entire BUB3 gene is approximately 16.2 Kb (start: 123154277 and end: 123170467 bp; orientation: Forward strand).
Transcription The BUB3 gene encodes for 4 transcript variants: there are two transcript variants deposited in the NCBI database ( and two additional transcript variants reported in Ensembl ( The transcript variant 1 is the longest transcript variant (exons: 8, coding exons: 7, transcript length: 7828 bp) and encodes the isoform a (328 amino acids [aa]). The transcript variant 2 present an alternative splice site in 3' coding region (exons: 8, coding exons: 7, transcript length: 1361 bp), which leads to a frameshift and a shorter and distinct C-terminus compared to isoform a (isoform b, 326 aa). The transcript variant 3 present 6 coding exons, a transcript length of 895 bp and a translation length of 278 aa. The transcript variant 4 is shorter transcript presenting 5 exons (being 4 coding exons), a transcript length 667 bp and resulting protein of 145 aa.


  Figure 1. BUB3 protein structure. BUB3 protein presents seven WD40 repeat domain, a nearly 40 amino acids (aa) motif rich in tryptophan-aspartic acids (W-D). The position of aa are indicated.
Description BUB3 is a WD40 protein that create a symmetric circular wall around a central pore or funnel region with its seven-blade β-propeller structure, acting as a scaffolding protein for its binding partners BUB1B (BUBR1) and BUB1 (Prinz et al., 2016; Seeley et al., 1999). The primary structure of BUB3 is illustrated in Figure 1.
Expression Ubiquitous.
Localisation BUB3 is localized preferentially in unattached kinetochores during mitosis (especially prometaphase), participating of mitotic checkpoint complex (Sudakin et al., 2001). In interphase cells, BUB3 is localized in cytosol (Yoon et al., 2004).
  Figure 2. BUB3, a component of mitotic checkpoint complex (MCC). (Upper panel) The presence of unattached kinotochores induces the formation of MCC composed by BUB3, BUBR1, MAD2L1, which sequester CDC20, leads the anaphase-promoting complex/cyclosome (APC/C) inhibition and cell cycle arrest. (Lower panel) In the presence of attached kinotochores, the MCC complex is disassembled and the CDC20 is released, which leads to APC/C activation (which in turn triggers degradation of securin and cyclin B), chromosomal segregation and completion of mitosis.
Function BUB3 protein is a component of the mitotic checkpoint complex (MCC), which present a crucial activity monitoring the state of chromosome attachment to the mitotic (or meiotic) fuse and prevents loss of sister chromatid cohesion and premature chromosome segregation in the presence of unattached or incorrectly attached chromosomes to the spindle by inhibition of the anaphase-promoting complex/cyclosome (APC/C) (Lopes et al., 2005; Musacchio, 2015). The canonical BUB3-related cellular signaling is illustrated in Figure 2.
Using a Drosophila melanogaster model, Morais da Silva and colleagues (Morais da Silva et al., 2013) reported that BUB3 inhibition resulted in increased proliferative potential, promoted tumorigenesis and widespread chromosomal aneuploidy. Interesting, loss of cytoplasmatic, but not attached-kinetochore, BUB3 pool was found to be driven tumorigenesis, indicating a novel non-kinetochore-dependent tumor suppressing function for BUB3 (Morais da Silva et al., 2013). Additional non-canonical functions of BUB3 had been described during interphase. BUB3 and CDC20 form a complex with histone deacetylases, which seems to confer transcriptional repressor activity (Yoon et al., 2004). Wan and colleagues (Wan et al., 2015) reported that BUB3 regulates RNA splicing, R-loop formation, DNA damage, and TP53 activation.
Homology The BUB3 gene and protein is highly homologous among different species, as shown in Table 1.
Table 1. Comparative identity of human BUB3 with other species.
% Identity for: Homo sapiens BUB3  SymbolProtein  DNA
vs. P.troglodytesBUB3100.099.8
vs. M.mulattaBUB3100.098.8
vs. B.taurusBUB3100.093.5
vs. M.musculusBub3100.090.4
vs. R.norvegicusBub3100.091.1
vs. G.gallusBUB396.984.0
vs. X.tropicalisbub393.279.5
vs. D.reriobub386.273.7
vs. D.melanogasterBub361.159.9
vs. A.gambiaeAgaP_AGAP01054462.059.2
vs. S.pombebub338.045.0
vs. A.thalianaBUB3.155.155.0
vs. A.thalianaBUB3.256.355.8
vs. O.sativaOs03g044860056.357.4



Somatic Recurrent mutations in the BUB3 gene are rare. A total of 81 unique samples presented BUB3 mutations, which are distributed on 75 different mutations (60 missense substitutions, 10 synonymous substitutions, 1 nonsense substitutions, 2 inframe deletions and 2 frameshift deletions), were found among the 47120 unique samples reported in COSMIC (Catalogue of Somatic Mutations in Cancer; In agreement, 0.2% of 74247 tested samples presented BUB3 somatic mutation as reported in cBioPortal (, which correspond to 154 mutations: 132 missense substitutions, 17 truncating and 5 inframe mutations. Of note, there are 55 duplicate mutations in patients with multiple samples. A total of 472 (0.8 %) samples presented any type of genetic alteration in BUB3, when mutations, amplifications, deep deletions and multiple alterations were considered in 55817 cancer samples. These findings corroborate initial studies in different types of cancer (Hernando et al., 2001).

Implicated in

Entity Adrenocortical carcinoma
Note In a cohort of 79 adrenocortical carcinoma patients, increased levels of BUB3 mRNA levels was associated with high grade tumors and poor clinical outcomes (Subramanian and Cohen, 2019). In addition, Oncomine Giordano ACC data analysis revealed increased levels of BUB3 in adrenocortical carcinoma compared to normal adrenal samples (Subramanian and Cohen, 2019).
Entity Breast cancer
Note BUB3 promoter polymorphisms (rs3763740, rs3763741, rs17014712, rs3808960 and rs3808961) did not impact familial breast cancer risk in a German cohort of 441 breast cancer patients and 552 controls matched by age, ethnicity and geographical region (Vaclavicek et al., 2007). Similarly, the polymorphisms rs11248416, rs11248419, and rs6599657 in BUB3 gene were not associated with risk of breast cancer development in a Chinese cohort of 462 breast cancer patients and 529 controls (Wang et al., 2014).
BUB3 was highly expressed in primary and cell lines from breast cancer compared to immortalized breast cancer epithelium or normal primary mammary cells (Yuan et al., 2006). BUB3 gene region (10q26.3) was found to be amplified and overexpressed in breast cancer samples (Turner et al., 2010). BUB3 mRNA levels were upregulated in doxorubicin and cyclophosphamide sensitive breast cancer tumors (Cleator et al., 2006). In MDA-MB-231 breast cancer cells, BUB3 expression was induced by BMP signaling (Yan et al., 2012).
Entity Cervical cancer
Note Using proteomics approach, BUB3 was found to be downregulated by paclitaxel and 5-fluorouracil in HeLa cells (Lee et al., 2005; Yim et al., 2004). In addition, BUB3 inhibition by siRNA reduced paclitaxel-induced cell cycle arrest (Lee et al., 2005).
Entity Chronic myeloid leukemia
Note BCR/ ABL1 expression repressed spindle checkpoint components, including BUB3, to escape from metaphase arrest (Wolanin et al., 2010).
Entity Colorectal cancer
Note Three missense variants in BUB3 [predicted to be pathogenic: c.790T>C (p.F264L), c.63G>C (p.K21N) and c.446G>A (p.R149Q)] were observed in familial or early onset colorectal cancer patients (n=185), which were not found in large cohort of control (n=1154) (de Voer et al., 2013). In agreement, Mur and colleagues (Mur et al., 2018) reported that BUB3 was found to be mutated [BUB3 c.77C>T (p.T26I)] in familial colorectal cancer.
Entity Gastric cancer
Note High BUB3 gene expression was observed in 34 out of 43 gastric cancer tumors samples compared with their matched normal mucosa counterpart, which was associated with Ki-67 expression, but not with aneuploidy (Grabsch et al., 2003). The BUB3 polymorphism, rs7897156, did not impact gastric cancer susceptibility in a study including 164 gastric cancer patients and 381 ethnicity matched controls (Mesic et al., 2017).
Entity Glioblastoma
Note he genetic variation C>T in the position -6 of the BUB3 gene, but not in coding sequence, was detected in 4 out of 22 glioblastoma samples (Reis et al., 2001). Bie and colleagues (Bie et al., 2011) reported that BUB3 was highly expressed in grade III and IV gliomas compared to normal brain samples. On the other hand, Morales and colleagues (Morales et al., 2013) reported a downregulation of BUB3 in primary samples and cell lines derived from glioblastoma compared to non-neoplastic white matter from epileptic patients.
In BUB3-depleted U87MG glioblastoma cells, the expression o BUB3Y207F, which mimics an unphosphorylated form, strongly reduced tumor growth and improved survival compared to the expression BUB3WT in intracranial tumor mice model, indicating that BUB3 phosphorylation at Y207 site is required for tumorigenesis (Jiang et al., 2014).
Entity Lung cancer
Note Initial observations did not identify genetic defects in BUB3 gene in lung cancer patients (Haruki et al., 2001). In cohort of 766 non-small cell lung cancer patients, the presence of allele T of polymorphism in BUB3 (rs7897156C>T) was associated with poor overall survival (Kang et al., 2017). Using H1299 and A549 non-small cell lung cell lines, functional studies based in luciferase assays indicated that T allele induced enhancement of BUB3 expression. In addition, increased BUB3 expression was observed in lung tumor tissues compared to non-malignant lung tissues (Kang et al., 2017).
Entity Osteosarcoma
Note Loss of heterozygosity at 10q26, but not BUB3 mutations, was found in ostesarcoma samples (Mendoza et al., 2005). In Saos-2 osteosarcoma cells, TAp73α (an isoform of TP73) binds to BUB3 and BUB1, which leads to potential alteration of mitotic checkpoint function of these proteins and aneuploidy (Vernole et al., 2009). In U2OS osteosarcoma cells, but not normal fibroblast, BUB3 silencing reduces cell proliferation and clonogenicity and induces DNA fragmentation (Prinz et al., 2016).
Entity Ovarian cystadenoma
Note In ML10 cells, a human ovarian cystadenoma model, BUB3 nuclear expression and phosphorylation were increased in low compared to high replicative age cells, which may be involved in prolonged mitotic arrest and cytokinesis failure observed in this cellular model (Austria et al., 2018).
Entity Pancreatic cancer
Note A mutation in BUB3 (c.576+1G>A) was found among deleterious germline mutations (n=33) in sporadic pancreatic adenocarcinoma patients (n=854) (Shindo et al., 2017). In pancreatic cells, DMAP1/BUB3 complex repressed antiapoptotic genes transcription mediating mitotic stress-induced apoptosis and improved in vivo paclitaxel-induced tumor growth inhibition, which are impaired by SRC activity (Li et al., 2018).
Entity Renal cell carcinoma
Note BUB3 gene expression profile was similar between samples from papillary renal cell carcinoma, chromophobe renal cell carcinoma and clear cell renal cell carcinoma patients and normal kidney tissues (Pinto et al., 2007; Pinto et al., 2008).

To be noted

BUB3 knockout mice presented accumulation of mitotic errors during embryogenesis and were unviable after day 6.5-7.5 postcoitus (Kalitsis et al., 2000). BUB3 haploinsufficiency leaded to chromosome instability and predisposition to carcinogen-induced, but not to spontaneous, tumors development in mice (Babu et al., 2003; Kalitsis et al., 2005; Rao et al., 2009). Induced-BUB3 mutations presented no effect on the genome rearrangements rate in Saccharomyces cerevisiae (Myung et al., 2001). In healthy humans, BUB3 genetic variations were not associated with non-specific chromosomal aberrations (Forsti et al., 2016).


Mechanism of cytokinesis failure in ovarian cystadenomas with defective BRCA1 and P53 pathways
Austria T, Marion C, Yu V, Widschwendter M, Hinton DR, Dubeau L
Int J Cancer 2018 Dec 1;143(11):2932-2942
PMID 29978915
Rae1 is an essential mitotic checkpoint regulator that cooperates with Bub3 to prevent chromosome missegregation
Babu JR, Jeganathan KB, Baker DJ, Wu X, Kang-Decker N, van Deursen JM
J Cell Biol 2003 Feb 3;160(3):341-53
PMID 12551952
The accuracy of survival time prediction for patients with glioma is improved by measuring mitotic spindle checkpoint gene expression
Bie L, Zhao G, Cheng P, Rondeau G, Porwollik S, Ju Y, Xia XQ, McClelland M
PLoS One 2011;6(10):e25631
PMID 22022424
Gene expression patterns for doxorubicin (Adriamycin) and cyclophosphamide (cytoxan) (AC) response and resistance
Cleator S, Tsimelzon A, Ashworth A, Dowsett M, Dexter T, Powles T, Hilsenbeck S, Wong H, Osborne CK, O'Connell P, Chang JC
Breast Cancer Res Treat 2006 Feb;95(3):229-33
PMID 16322899
Genetic variation in the major mitotic checkpoint genes associated with chromosomal aberrations in healthy humans
Försti A, Frank C, Smolkova B, Kazimirova A, Barancokova M, Vymetalkova V, Kroupa M, Naccarati A, Vodickova L, Buchancova J, Dusinska M, Musak L, Vodicka P, Hemminki K
Cancer Lett 2016 Oct 1;380(2):442-6
PMID 27424524
Overexpression of the mitotic checkpoint genes BUB1, BUBR1, and BUB3 in gastric cancer--association with tumour cell proliferation
Grabsch H, Takeno S, Parsons WJ, Pomjanski N, Boecking A, Gabbert HE, Mueller W
J Pathol 2003 May;200(1):16-22
PMID 12692836
Molecular analysis of the mitotic checkpoint genes BUB1, BUBR1 and BUB3 in human lung cancers
Haruki N, Saito H, Harano T, Nomoto S, Takahashi T, Osada H, Fujii Y, Takahashi T
Cancer Lett 2001 Jan 26;162(2):201-5
PMID 11146226
Molecular analyses of the mitotic checkpoint components hsMAD2, hBUB1 and hBUB3 in human cancer
Hernando E, Orlow I, Liberal V, Nohales G, Benezra R, Cordon-Cardo C
Int J Cancer 2001 Jul 20;95(4):223-7
PMID 11400114
PKM2 regulates chromosome segregation and mitosis progression of tumor cells
Jiang Y, Li X, Yang W, Hawke DH, Zheng Y, Xia Y, Aldape K, Wei C, Guo F, Chen Y, Lu Z
Mol Cell 2014 Jan 9;53(1):75-87
PMID 24316223
Increased chromosome instability but not cancer predisposition in haploinsufficient Bub3 mice
Kalitsis P, Fowler KJ, Griffiths B, Earle E, Chow CW, Jamsen K, Choo KH
Genes Chromosomes Cancer 2005 Sep;44(1):29-36
PMID 15898111
Polymorphisms in mitotic checkpoint-related genes can influence survival outcomes of early-stage non-small cell lung cancer
Kang HG, Yoo SS, Choi JE, Hong MJ, Do SK, Jin CC, Kim S, Lee WK, Choi SH, Lee SY, Kim HJ, Lee SY, Lee J, Cha SI, Kim CH, Seok Y, Lee E, Cho S, Jheon S, Park JY
Oncotarget 2017 Jun 27;8(37):61777-61785
PMID 28977903
Proteomic analysis of anti-cancer effects by paclitaxel treatment in cervical cancer cells
Lee KH, Yim EK, Kim CJ, Namkoong SE, Um SJ, Park JS
Gynecol Oncol 2005 Jul;98(1):45-53
PMID 15907983
C-Src confers resistance to mitotic stress through inhibition DMAP1/Bub3 complex formation in pancreatic cancer
Li J, Hu B, Wang T, Huang W, Ma C, Zhao Q, Zhuo L, Zhang T, Jiang Y
Mol Cancer 2018 Dec 15;17(1):174
PMID 30553276
The Drosophila Bub3 protein is required for the mitotic checkpoint and for normal accumulation of cyclins during G2 and early stages of mitosis
Lopes CS, Sampaio P, Williams B, Goldberg M, Sunkel CE
J Cell Sci 2005 Jan 1;118(Pt 1):187-98
PMID 15615783
Allelic loss at 10q26 in osteosarcoma in the region of the BUB3 and FGFR2 genes
Mendoza S, David H, Gaylord GM, Miller CW
Cancer Genet Cytogenet 2005 Apr 15;158(2):142-7
PMID 15796961
Single nucleotide polymorphisms rs911160 in AURKA and rs2289590 in AURKB mitotic checkpoint genes contribute to gastric cancer susceptibility
Mesic A, Markocic E, Rogar M, Juvan R, Hudler P, Komel R
Environ Mol Mutagen 2017 Dec;58(9):701-711
PMID 28843004
A tumor suppressor role of the Bub3 spindle checkpoint protein after apoptosis inhibition
Morais da Silva S, Moutinho-Santos T, Sunkel CE
J Cell Biol 2013 Apr 29;201(3):385-93
BUB1 and BUBR1 inhibition decreases proliferation and colony formation, and enhances radiation sensitivity in pediatric glioblastoma cells
Morales AG, Pezuk JA, Brassesco MS, de Oliveira JC, de Paula Queiroz RG, Machado HR, Carlotti CG Jr, Neder L, de Oliveira HF, Scrideli CA, Tone LG
Childs Nerv Syst 2013 Dec;29(12):2241-8
Germline mutations in the spindle assembly checkpoint genes BUB1 and BUB3 are infrequent in familial colorectal cancer and polyposis
Mur P, De Voer RM, Olivera-Salguero R, Rodríguez-Perales S, Pons T, Setién F, Aiza G, Valdés-Mas R, Bertini A, Pineda M, Vreede L, Navarro M, Iglesias S, González S, Brunet J, Valencia A, Esteller M, Lázaro C, Kops GJPL, Urioste M, Puente XS, Capellá G, Valle L
Mol Cancer 2018 Feb 15;17(1):23
PMID 29448935
The Molecular Biology of Spindle Assembly Checkpoint Signaling Dynamics
Musacchio A
Curr Biol 2015 Oct 19;25(20):R1002-18
PMID 26485365
Suppression of spontaneous chromosomal rearrangements by S phase checkpoint functions in Saccharomyces cerevisiae
Myung K, Datta A, Kolodner RD
Cell 2001 Feb 9;104(3):397-408
PMID 11239397
Expression changes of the MAD mitotic checkpoint gene family in renal cell carcinomas characterized by numerical chromosome changes
Pinto M, Soares MJ, Cerveira N, Henrique R, Ribeiro FR, Oliveira J, Jerónimo C, Teixeira MR
Virchows Arch 2007 Apr;450(4):379-85
PMID 17333263
Overexpression of the mitotic checkpoint genes BUB1 and BUBR1 is associated with genomic complexity in clear cell kidney carcinomas
Pinto M, Vieira J, Ribeiro FR, Soares MJ, Henrique R, Oliveira J, Jerónimo C, Teixeira MR
Cell Oncol 2008;30(5):389-95
PMID 18791270
Functional and Structural Characterization of Bub3·BubR1 Interactions Required for Spindle Assembly Checkpoint Signaling in Human Cells
Prinz F, Puetter V, Holton SJ, Andres D, Stegmann CM, Kwiatkowski D, Prechtl S, Petersen K, Beckmann G, Kreft B, Mumberg D, Fernández-Montalván A
J Biol Chem 2016 May 20;291(21):11252-67
PMID 27030009
Enhanced genomic instabilities caused by deregulated microtubule dynamics and chromosome segregation: a perspective from genetic studies in mice
Rao CV, Yamada HY, Yao Y, Dai W
Carcinogenesis 2009 Sep;30(9):1469-74
PMID 19372138
Mutation analysis of hBUB1, hBUBR1 and hBUB3 genes in glioblastomas
Reis RM, Nakamura M, Masuoka J, Watanabe T, Colella S, Yonekawa Y, Kleihues P, Ohgaki H
Acta Neuropathol 2001 Apr;101(4):297-304
PMID 11355300
Phosphorylation of human MAD1 by the BUB1 kinase in vitro
Seeley TW, Wang L, Zhen JY
Biochem Biophys Res Commun 1999 Apr 13;257(2):589-95
PMID 10198256
Deleterious Germline Mutations in Patients With Apparently Sporadic Pancreatic Adenocarcinoma
Shindo K, Yu J, Suenaga M, Fesharakizadeh S, Cho C, Macgregor-Das A, Siddiqui A, Witmer PD, Tamura K, Song TJ, Navarro Almario JA, Brant A, Borges M, Ford M, Barkley T, He J, Weiss MJ, Wolfgang CL, Roberts NJ, Hruban RH, Klein AP, Goggins M
J Clin Oncol 2017 Oct 20;35(30):3382-3390
PMID 28767289
Over expression of DNA damage and cell cycle dependent proteins are associated with poor survival in patients with adrenocortical carcinoma
Subramanian C, Cohen MS
Surgery 2019 Jan;165(1):202-210
PMID 30413320
Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2
Sudakin V, Chan GK, Yen TJ
J Cell Biol 2001 Sep 3;154(5):925-36
PMID 11535616
Integrative molecular profiling of triple negative breast cancers identifies amplicon drivers and potential therapeutic targets
Turner N, Lambros MB, Horlings HM, Pearson A, Sharpe R, Natrajan R, Geyer FC, van Kouwenhove M, Kreike B, Mackay A, Ashworth A, van de Vijver MJ, Reis-Filho JS
Oncogene 2010 Apr 8;29(14):2013-23
PMID 20101236
Genetic variation in the major mitotic checkpoint genes does not affect familial breast cancer risk
Vaclavicek A, Bermejo JL, Wappenschmidt B, Meindl A, Sutter C, Schmutzler RK, Kiechle M, Bugert P, Burwinkel B, Bartram CR, Hemminki K, Försti A
Breast Cancer Res Treat 2007 Dec;106(2):205-13
PMID 17268814
TAp73alpha binds the kinetochore proteins Bub1 and Bub3 resulting in polyploidy
Vernole P, Neale MH, Barcaroli D, Munarriz E, Knight RA, Tomasini R, Mak TW, Melino G, De Laurenzi V
Cell Cycle 2009 Feb 1;8(3):421-9
PMID 19182530
Splicing function of mitotic regulators links R-loop-mediated DNA damage to tumor cell killing
Wan Y, Zheng X, Chen H, Guo Y, Jiang H, He X, Zhu X, Zheng Y
J Cell Biol 2015 Apr 27;209(2):235-46
PMID 25918225
Genetic variation in the major mitotic checkpoint genes and risk of breast cancer: a multigenic study on cancer susceptibility
Wang P, Wang Y, Yan H, Xie Q, Zhao L, Xu S, Zhao Q
Tumour Biol 2014 Jul;35(7):6701-5
PMID 24711138
Expression of oncogenic kinase Bcr-Abl impairs mitotic checkpoint and promotes aberrant divisions and resistance to microtubule-targeting agents
Wolanin K, Magalska A, Kusio-Kobialka M, Podszywalow-Bartnicka P, Vejda S, McKenna SL, Mosieniak G, Sikora E, Piwocka K
Mol Cancer Ther 2010 May;9(5):1328-38
PMID 20442314
Bone morphogenetic protein (BMP) signaling regulates mitotic checkpoint protein levels in human breast cancer cells
Yan H, Zhu S, Song C, Liu N, Kang J
Cell Signal 2012 Apr;24(4):961-8
PMID 22234345
Proteomic analysis of antiproliferative effects by treatment of 5-fluorouracil in cervical cancer cells
Yim EK, Lee KH, Bae JS, Namkoong SE, Um SJ, Park JS
DNA Cell Biol 2004 Nov;23(11):769-76
PMID 15585135
WD repeat-containing mitotic checkpoint proteins act as transcriptional repressors during interphase
Yoon YM, Baek KH, Jeong SJ, Shin HJ, Ha GH, Jeon AH, Hwang SG, Chun JS, Lee CW
FEBS Lett 2004 Sep 24;575(1-3):23-9
Increased expression of mitotic checkpoint genes in breast cancer cells with chromosomal instability
Yuan B, Xu Y, Woo JH, Wang Y, Bae YK, Yoon DS, Wersto RP, Tully E, Wilsbach K, Gabrielson E
Clin Cancer Res 2006 Jan 15;12(2):405-10
PMID 16428479
Germline mutations in the spindle assembly checkpoint genes BUB1 and BUB3 are risk factors for colorectal cancer
de Voer RM, Geurts van Kessel A, Weren RD, Ligtenberg MJ, Smeets D, Fu L, Vreede L, Kamping EJ, Verwiel ET, Hahn MM, Ariaans M, Spruijt L, van Essen T, Houge G, Schackert HK, Sheng JQ, Venselaar H, van Ravenswaaij-Arts CM, van Krieken JH, Hoogerbrugge N, Kuiper RP
Gastroenterology 2013 Sep;145(3):544-7
PMID 23747338


This paper should be referenced as such :
Jorge Antonio Elias Godoy Carlos, João Agostinho Machado-Neto
BUB3 (BUB3 mitotic checkpoint protein)
Atlas Genet Cytogenet Oncol Haematol. 2020;24(1):33-38.
Free journal version : [ pdf ]   [ DOI ]

External links


HGNC (Hugo)BUB3   1151
Atlas Explorer : (Salamanque)BUB3
Entrez_Gene (NCBI)BUB3    BUB3 mitotic checkpoint protein
AliasesBUB3L; hBUB3
GeneCards (Weizmann)BUB3
Ensembl hg19 (Hinxton)ENSG00000154473 [Gene_View]
Ensembl hg38 (Hinxton)ENSG00000154473 [Gene_View]  ENSG00000154473 [Sequence]  chr10:123154402-123170467 [Contig_View]  BUB3 [Vega]
ICGC DataPortalENSG00000154473
TCGA cBioPortalBUB3
AceView (NCBI)BUB3
Genatlas (Paris)BUB3
SOURCE (Princeton)BUB3
Genetics Home Reference (NIH)BUB3
Genomic and cartography
GoldenPath hg38 (UCSC)BUB3  -     chr10:123154402-123170467 +  10q26.13   [Description]    (hg38-Dec_2013)
GoldenPath hg19 (UCSC)BUB3  -     10q26.13   [Description]    (hg19-Feb_2009)
GoldenPathBUB3 - 10q26.13 [CytoView hg19]  BUB3 - 10q26.13 [CytoView hg38]
Genome Data Viewer NCBIBUB3 [Mapview hg19]  
Gene and transcription
Genbank (Entrez)AF047472 AF047473 AF053304 AF081496 AK226060
RefSeq transcript (Entrez)NM_001007793 NM_004725
Consensus coding sequences : CCDS (NCBI)BUB3
Gene ExpressionBUB3 [ NCBI-GEO ]   BUB3 [ EBI - ARRAY_EXPRESS ]   BUB3 [ SEEK ]   BUB3 [ MEM ]
Gene Expression Viewer (FireBrowse)BUB3 [ Firebrowse - Broad ]
GenevisibleExpression of BUB3 in : [tissues]  [cell-lines]  [cancer]  [perturbations]  
BioGPS (Tissue expression)9184
GTEX Portal (Tissue expression)BUB3
Human Protein AtlasENSG00000154473-BUB3 [pathology]   [cell]   [tissue]
Protein : pattern, domain, 3D structure
UniProt/SwissProtO43684   [function]  [subcellular_location]  [family_and_domains]  [pathology_and_biotech]  [ptm_processing]  [expression]  [interaction]
NextProtO43684  [Sequence]  [Exons]  [Medical]  [Publications]
With graphics : InterProO43684
Domaine pattern : Prosite (Expaxy)WD_REPEATS_2 (PS50082)    WD_REPEATS_REGION (PS50294)   
Domains : Interpro (EBI)G-protein_beta_WD-40_rep    WD40/YVTN_repeat-like_dom_sf    WD40_repeat    WD40_repeat_dom    WD40_repeat_dom_sf   
Domain families : Pfam (Sanger)WD40 (PF00400)   
Domain families : Pfam (NCBI)pfam00400   
Domain families : Smart (EMBL)WD40 (SM00320)  
Conserved Domain (NCBI)BUB3
AlphaFold pdb e-kbO43684   
Human Protein Atlas [tissue]ENSG00000154473-BUB3 [tissue]
Protein Interaction databases
IntAct (EBI)O43684
Ontologies - Pathways
Ontology : AmiGOmitotic sister chromatid segregation  kinetochore  kinetochore  protein binding  nucleoplasm  cytosol  mitotic spindle assembly checkpoint signaling  attachment of spindle microtubules to kinetochore  mitotic checkpoint complex  mitotic checkpoint complex  protein localization to kinetochore  ubiquitin binding  cell division  meiotic cell cycle  negative regulation of ubiquitin-protein transferase activity  bub1-bub3 complex  
Ontology : EGO-EBImitotic sister chromatid segregation  kinetochore  kinetochore  protein binding  nucleoplasm  cytosol  mitotic spindle assembly checkpoint signaling  attachment of spindle microtubules to kinetochore  mitotic checkpoint complex  mitotic checkpoint complex  protein localization to kinetochore  ubiquitin binding  cell division  meiotic cell cycle  negative regulation of ubiquitin-protein transferase activity  bub1-bub3 complex  
REACTOMEO43684 [protein]
REACTOME PathwaysR-HSA-68877 [pathway]   
NDEx NetworkBUB3
Atlas of Cancer Signalling NetworkBUB3
Wikipedia pathwaysBUB3
Orthology - Evolution
GeneTree (enSembl)ENSG00000154473
Phylogenetic Trees/Animal Genes : TreeFamBUB3
Homologs : HomoloGeneBUB3
Homology/Alignments : Family Browser (UCSC)BUB3
Gene fusions - Rearrangements
Fusion : QuiverBUB3
Polymorphisms : SNP and Copy number variants
NCBI Variation ViewerBUB3 [hg38]
dbSNP Single Nucleotide Polymorphism (NCBI)BUB3
Exome Variant ServerBUB3
GNOMAD BrowserENSG00000154473
Varsome BrowserBUB3
ACMGBUB3 variants
Genomic Variants (DGV)BUB3 [DGVbeta]
DECIPHERBUB3 [patients]   [syndromes]   [variants]   [genes]  
CONAN: Copy Number AnalysisBUB3 
ICGC Data PortalBUB3 
TCGA Data PortalBUB3 
Broad Tumor PortalBUB3
OASIS PortalBUB3 [ Somatic mutations - Copy number]
Somatic Mutations in Cancer : COSMICBUB3  [overview]  [genome browser]  [tissue]  [distribution]  
Somatic Mutations in Cancer : COSMIC3DBUB3
Mutations and Diseases : HGMDBUB3
LOVD (Leiden Open Variation Database)[gene] [transcripts] [variants]
DgiDB (Drug Gene Interaction Database)BUB3
DoCM (Curated mutations)BUB3
CIViC (Clinical Interpretations of Variants in Cancer)BUB3
Impact of mutations[PolyPhen2] [Provean] [Buck Institute : MutDB] [Mutation Assessor] [Mutanalyser]
Genetic Testing Registry BUB3
NextProtO43684 [Medical]
Target ValidationBUB3
Huge Navigator BUB3 [HugePedia]
Clinical trials, drugs, therapy
Protein Interactions : CTDBUB3
Pharm GKB GenePA25467
Clinical trialBUB3
DataMed IndexBUB3
PubMed179 Pubmed reference(s) in Entrez
GeneRIFsGene References Into Functions (Entrez)
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed

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© Atlas of Genetics and Cytogenetics in Oncology and Haematology
indexed on : Wed Jan 19 18:43:11 CET 2022

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