| |  |
| |
| | R1, R2 and R3 form DNA-binding domain, TA - transactivation domain, CR - conserved region, RD - negative regulatory domain. |
| |
| Description | 752 amino acids, 95 kDa protein.
R1, R2, R3 - three repeats 50 amino acids long, R2 and R3 contain HTH (helix-turn-helix) motives with unconventional turns required for DNA-binding activity, R1 serves as a DNA/protein complex stabilizer;
TA contains acidic amino acids and responsible for transcriptional activation;
CR known as an inhibitor of specific DNA binding activity;
RD is responsible for repression of transactivation function of A-MYB. |
| Expression | Mainly in spermatocytes, neuronal cells and B-lymphoid cells. Also was reported to be expressed in uterine cells and B-cells germinal centres. |
| Localisation | Nucleus. |
| Function | Transcription factor which play a role in the proliferation and differentiation of neurogenic, spermatogenic and B-lymphoid cells. A-MYB knockout mice demonstrated growth abnormalities, and defects in spermatogenesis and female breast development, whereas constantly high expression level of A-MYB in transgenic mice caused hyperplasia in the lymph nodes and spleen. |
| Homology | A-MYB is a member of MYB transcription factors family, which includes C-MYB and B-MYB, with high homology within TA and RD regions. DNA-binding domain is almost identical with that of B-MYB and C-MYB and conserved between mouse, human, drosophila and chicken. |
| Chromosome locations of the MYB related genes, AMYB and BMYB. |
| Barletta C, Druck T, LaForgia S, Calabretta B, Drabkin H, Patterson D, Croce CM, Huebner K. |
| Cancer Res. 1991 Jul 15;51(14):3821-4. |
| PMID 2065336 |
| |
| DNA binding and transactivation activity of A-myb, a c-myb-related gene. |
| Ma XP, Calabretta B. |
| Cancer Res. 1994 Dec 15;54(24):6512-6. |
| PMID 7987850 |
| |
| Expression of A-myb, but not c-myb and B-myb, is restricted to Burkitt's lymphoma, sIg+ B-acute lymphoblastic leukemia, and a subset of chronic lymphocytic leukemias. |
| Golay J, Luppi M, Songia S, Palvarini C, Lombardi L, Aiello A, Delia D, Lam K, Crawford DH, Biondi A, Barbui T, Rambaldi A, Introna M. |
| Blood. 1996 Mar 1;87(5):1900-11. |
| PMID 8634438 |
| |
| Ectopic expression of A-myb in transgenic mice causes follicular hyperplasia and enhanced B lymphocyte proliferation. |
| DeRocco SE, Iozzo R, Ma XP, Schwarting R, Peterson D, Calabretta B. |
| Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3240-4. |
| PMID 9096377 |
| |
| Arrest of spermatogenesis and defective breast development in mice lacking A-myb. |
| Toscani A, Mettus RV, Coupland R, Simpkins H, Litvin J, Orth J, Hatton KS, Reddy EP. |
| Nature. 1997 Apr 17;386(6626):713-7. |
| PMID 9109487 |
| |
| Linking Myb to the cell cycle: cyclin-dependent phosphorylation and regulation of A-Myb activity. |
| Ziebold U, Klempnauer KH. |
| Oncogene. 1997 Aug 28;15(9):1011-9. |
| PMID 9285555 |
| |
| The A-Myb transcription factor is a marker of centroblasts in vivo. |
| Golay J, Broccoli V, Lamorte G, Bifulco C, Parravicini C, Pizzey A, Thomas NS, Delia D, Ferrauti P, Vitolo D, Introna M. |
| J Immunol. 1998 Mar 15;160(6):2786-93. |
| PMID 9510180 |
| |
| The myb gene family in cell growth, differentiation and apoptosis. |
| Oh IH, Reddy EP. |
| Oncogene. 1999 May 13;18(19):3017-33. Review |
| PMID 10378697 |
| |
| A-Myb up-regulates Bcl-2 through a Cdx binding site in t(14;18) lymphoma cells. |
| Heckman CA, Mehew JW, Ying GG, Introna M, Golay J, Boxer LM. |
| J Biol Chem. 2000 Mar 3;275(9):6499-508. |
| PMID 10692454 |
| |
| Estrogen-induced changes in IGF-I, Myb family and MAP kinase pathway genes in human uterine leiomyoma and normal uterine smooth muscle cell lines. |
| Swartz CD, Afshari CA, Yu L, Hall KE, Dixon D. |
| Mol Hum Reprod. 2005 Jun;11(6):441-50. Epub 2005 May 6. |
| PMID 15879465 |
| |
