Atlas of Genetics and Cytogenetics in Oncology and Haematology


Home   Genes   Leukemias   Solid Tumours   Cancer-Prone   Deep Insight   Case Reports   Journals  Portal   Teaching   

X Y 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 NA

Soft Tissues: Myxoinflammatory fibroblastic sarcoma with t(1;10)(p22;q24) FGF8/

Written2012-02Karolin H Nord
Department of Clinical Genetics, University, Regional Laboratories, Skane University Hospital, Lund University, Lund, Sweden

(Note : for Links provided by Atlas : click)

Identity

ICD-Topo C470-C476,C478-C479,C490-C496,C498-C499 CONNECTIVE & SOFT TISSUE
Atlas_Id 6369
Phylum Soft tissues Tumors:Uncertain differentiation:Myxoinflammatory fibroblastic sarcoma:t(1;10)(p22;q24)
Other namesMIFS was originally described as acral MIFS (Meis-Kindblom and Kindblom, 1998).
Note MIFS is an intermediate malignant soft tissue tumor usually located in the subcutaneous tissue of distal extremities (Kindblom et al., 2002). Distant metastases are rare but the tumor has a propensity for multiple local recurrences. An identical t(1;10)(p22;q24) has been found in MIFS and hemosiderotic fibrolipomatous tumor (HFLT) (Hallor et al., 2009; Antonescu et al., 2011). HFLT is an intermediate malignant tumor of uncertain differentiation, morphologically distinct from MIFS. In similarity with MIFS, HFLT has a predilection for superficial soft tissue of distal extremities and present frequent local recurrences. Despite their usually distinct morphology, there are tumors with mixed HFLT/MIFS histology (Elco et al., 2010). These tumors also show a t(1;10) and suggest that there are either different morphological variants or different levels of tumor progression of a sole biological entity (Antonescu et al., 2011).

Classification

Note MIFS is an intermediate malignant fibroblastic/myofibroblastic soft tissue tumor.

Clinics and Pathology

Disease Myxoinflammatory fibroblastic sarcoma (MIFS)
Phenotype / cell stem origin The origin of the tumor cells is unknown. Their fibroblastic/myofibroblastic differentiation indicates that they derive from a mesenchymal precursor.
Etiology Unknown.
Epidemiology MIFS is a rare soft tissue tumor which primarily affects adults without any gender predilection.
Clinics MIFS is an intermediate malignant tumor that usually presents as a slowly-growing, poorly-delineated mass of the superficial soft tissue of distal extremities (Kindblom et al., 2002). It is sometimes associated with pain and decreased mobility. In many cases the growth has been noted for a relatively long period of time before diagnosis. MIFS may be confused with inflammatory or post-traumatic lesions, benign or other malignant soft tissue tumors. Local recurrences are common but distant metastases are very rare.
Pathology MIFS show a poorly delineated, multinodular growth pattern with alternating myxoid and cellular areas (Kindblom et al., 1998). There is a prominent inflammatory infiltrate that may obscure the neoplastic cells and cause misdiagnoses of a reactive or inflammatory process. Tumor cells, including large polygonal and bizarre ganglion-like cells with prominent inclusion-like nucleoli and variably sized, multivacuolated lipoblast-like cells, may be scattered singly or form coherent clusters.
Treatment The treatment for MIFS is surgical excision.
Prognosis Local recurrences are common and their incidence may depend on primary surgical treatment; multiple local recurrences may require eventual amputation (Kindblom et al., 2002). Distant metastases are, however, exceedingly rare.

Genetics

Note MIFS, HFLT and tumors with mixed MIFS/HFLT histology share the same genetic aberrations; t(1;10) and amplification of a region in proximal chromosome arm 3p (Antonescu et al., 2011).

Cytogenetics

Cytogenetics
Morphological
The majority of cytogenetically analyzed MIFS present a t(1;10)(p22;q24), or variants thereof (Mitelman Database of Chromosome Aberrations in Cancer 2012). Ring and/or giant marker chromosomes as well as aberrations involving chromosome 3 are also associated with this disease.
Partial karyotype with a t(1;10)(p22;q24) and rearrangement of 3p.
Cytogenetics Molecular Fluorescence in situ hybridization analyses, using probes flanking the genes TGFBR3 in chromosome 1 and MGEA5 in chromosome 10, can be used as a diagnostic molecular test for MIFS and HFLT (Antonescu et al., 2011). Amplification of material from chromosome arm 3p can be detected by fluorescence in situ hybridization analyses and/or array-based genomic copy number analyses (Hallor et al., 2009).
Fluorescence in situ hybridization, using probes flanking the TGFBR3 and MGEA5 genes, respectively, can be used to detect the t(1;10)(p22;q24). A normal chromosome 10 show signals from probes located on either side of MGEA5 (labeled in red and yellow). On the der(10)t(1;10) the proximal probe (yellow) is detected while the more distal probe (red) is deleted.

Genes involved and Proteins

Note The breaks in chromosomes 1 and 10 seem to occur in, or close to, the genes TGFBR3 and MGEA5, respectively, and the translocation juxtaposes FGF8 in chromosome 10 with TGFBR3 in chromosome 1 (Hallor et al., 2009). FGF8 is highly expressed, likely as a result of the rearrangement, in tumors affected by the translocation. Ring and giant marker chromosomes in MIFS contain amplified material from chromosome 3. The core amplicon harbors the gene VGLL3, which is also highly expressed in affected tumors (Hallor et al., 2009). This abnormality is, however, not specific for MIFS/HFLT and have been found in additional sarcomas as well as other malignancies (Hélias-Rodzewicz et al., 2010).
Gene Name VGLL3
Location 3p12
Note Amplification and high expression of VGLL3 in chromosome 3 is found in MIFS and HFLT as well as other sarcomas.
Dna / Rna VGLL3 is a protein coding gene located at position 86987119-87040269 in chromosome 3 (http://www.ensembl.org; human assembly GRCh37). There are three transcript variants of this gene. The most extensive variant (transcript variant 1) comprises 10400 base pairs and consists of 4 coding exons.
DNA copy number analysis of a MIFS using array comparative genomic hybridization. A genome-wide copy number profile displays tumor/reference log2 ratios across the genome (top). Individual chromosomes are separated by vertical bars and chromosome 3 is labeled in yellow. The profile shows amplification of material from chromosome 3 and a few additional aberrations. Enlarged view of chromosome 3 shows two separate amplicons, the more proximal of these contains the VGLL3 gene (bottom).
Protein VGLL3 encodes the protein vestigial like 3 (Drosophila). Translation of VGLL3 transcript variant 1 results in a 326 amino acid protein. There is not much known about the function of this protein. However, it is believed that mammlian vestigal like proteins could be involved in regulating members of the TEAD transcription factor family (Vaudin et al., 1999; Maeda et al., 2002).

Gene Name FGF8
Location 10q24
Note The translocation between chromosomes 1 and 10 juxtaposes FGF8 in chromosome 10 with TGFBR3 in chromosome 1. In tumors affected by the translocation, FGF8 is highly expressed.
Dna / Rna FGF8 is a highly conserved gene located at position 103530081-103535827 in chromosome 10 (http://www.ensembl.org; human assembly GRCh37). There are six transcript variants of this gene and the alternating splicing results in products of 4-6 exons, which in turn encode proteins of 204-244 amino acids. The expression of FGF8 is controlled by several regulatory sequences located both upstream and downstream of the gene (Beermann et al., 2006; Inoue et al., 2006).
Protein Fibroblast growth factor 8 belongs to the large family of fibroblast growth factors. Members of this family are secreted molecules which by activating their receptors are involved in a variety of biological processes (Thisse and Thisse et al., 2005). FGF8 is transcriptionally silent in most normal adult tissues. However, upregulation of this gene has been associated with tumor growth and has been identified in carcinomas of the breast, prostate, and ovary, as well as in synovial sarcoma (Tanaka et al., 1998; Ishibe et al., 2005; Mattila et al., 2007).

Result of the chromosomal anomaly

Hybrid Gene
Note The t(1;10) juxtaposes FGF8 in chromosome 10 with TGFBR3 in chromosome 1 and the rearrangement is associated with high expression of FGF8 (Hallor et al., 2009).
 
Fluorescence in situ hybridization analyses suggest that TGFBR3 is translocated from chromosome 1 and positioned in opposite direction next to the MGEA5 gene on the der(10)t(1;10) (Hallor et al., 2009).
Description The translocation does not result in a conventional fusion gene. The functional outcome of the recurrent aberration seems to be high expression of the gene FGF8 (Hallor et al., 2009). The consistent involvement of TGFBR3, but lack of fusion transcripts, suggest that regulatory sequences in TGFBR3 are crucial for malignant transformation.
Transcript Rearrangements of TGFBR3 and MGEA5.
Detection Fluorescence in situ hybridization analysis using probes flanking the TGFBR3 and MGEA5 genes can be applied as a molecular test for detecting rearrangements of the genes (Antonescu et al., 2011).
  

Bibliography

Consistent t(1;10) with rearrangements of TGFBR3 and MGEA5 in both myxoinflammatory fibroblastic sarcoma and hemosiderotic fibrolipomatous tumor.
Antonescu CR, Zhang L, Nielsen GP, Rosenberg AE, Dal Cin P, Fletcher CD.
Genes Chromosomes Cancer. 2011 Oct;50(10):757-64. doi: 10.1002/gcc.20897. Epub 2011 Jun 29.
PMID 21717526
 
Identification of evolutionarily conserved regulatory elements in the mouse Fgf8 locus.
Beermann F, Kaloulis K, Hofmann D, Murisier F, Bucher P, Trumpp A.
Genesis. 2006 Jan;44(1):1-6.
PMID 16397882
 
Hybrid myxoinflammatory fibroblastic sarcoma/hemosiderotic fibrolipomatous tumor: report of a case providing further evidence for a pathogenetic link.
Elco CP, Marino-Enriquez A, Abraham JA, Dal Cin P, Hornick JL.
Am J Surg Pathol. 2010 Nov;34(11):1723-7.
PMID 20871391
 
Two genetic pathways, t(1;10) and amplification of 3p11-12, in myxoinflammatory fibroblastic sarcoma, haemosiderotic fibrolipomatous tumour, and morphologically similar lesions.
Hallor KH, Sciot R, Staaf J, Heidenblad M, Rydholm A, Bauer HC, Astrom K, Domanski HA, Meis JM, Kindblom LG, Panagopoulos I, Mandahl N, Mertens F.
J Pathol. 2009 Apr;217(5):716-27.
PMID 19199331
 
YAP1 and VGLL3, encoding two cofactors of TEAD transcription factors, are amplified and overexpressed in a subset of soft tissue sarcomas.
Helias-Rodzewicz Z, Perot G, Chibon F, Ferreira C, Lagarde P, Terrier P, Coindre JM, Aurias A.
Genes Chromosomes Cancer. 2010 Dec;49(12):1161-71.
PMID 20842732
 
Genomic organization, alternative splicing, and multiple regulatory regions of the zebrafish fgf8 gene.
Inoue F, Nagayoshi S, Ota S, Islam ME, Tonou-Fujimori N, Odaira Y, Kawakami K, Yamasu K.
Dev Growth Differ. 2006 Sep;48(7):447-62.
PMID 16961592
 
Disruption of fibroblast growth factor signal pathway inhibits the growth of synovial sarcomas: potential application of signal inhibitors to molecular target therapy.
Ishibe T, Nakayama T, Okamoto T, Aoyama T, Nishijo K, Shibata KR, Shima Y, Nagayama S, Katagiri T, Nakamura Y, Nakamura T, Toguchida J.
Clin Cancer Res. 2005 Apr 1;11(7):2702-12.
PMID 15814652
 
Myxoinflammatory fibroblastic sarcoma.
Kindblom LG, Meis-Kindblom JM.
In Fletcher CDM, Unni KK, Mertens F (Eds.). World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of Soft Tissue and Bone. Lyon: IARC Press, 2002.
 
Mammalian vestigial-like 2, a cofactor of TEF-1 and MEF2 transcription factors that promotes skeletal muscle differentiation.
Maeda T, Chapman DL, Stewart AF.
J Biol Chem. 2002 Dec 13;277(50):48889-98. Epub 2002 Oct 9.
PMID 12376544
 
Role of fibroblast growth factor 8 in growth and progression of hormonal cancer.
Mattila MM, Harkonen PL.
Cytokine Growth Factor Rev. 2007 Jun-Aug;18(3-4):257-66. Epub 2007 May 23. (REVIEW)
PMID 17512240
 
Acral myxoinflammatory fibroblastic sarcoma: a low-grade tumor of the hands and feet.
Meis-Kindblom JM, Kindblom LG.
Am J Surg Pathol. 1998 Aug;22(8):911-24.
PMID 9706971
 
Mitelman Database of Chromosome Aberrations and Gene Fusions in Cancer.
Mitelman F, Johansson B, Mertens F.
Mitelman F, Johansson B and Mertens F (Eds.), 2012. http://cgap.nci.nih.gov/Chromosomes/Mitelman
 
High frequency of fibroblast growth factor (FGF) 8 expression in clinical prostate cancers and breast tissues, immunohistochemically demonstrated by a newly established neutralizing monoclonal antibody against FGF 8.
Tanaka A, Furuya A, Yamasaki M, Hanai N, Kuriki K, Kamiakito T, Kobayashi Y, Yoshida H, Koike M, Fukayama M.
Cancer Res. 1998 May 15;58(10):2053-6.
PMID 9605740
 
Functions and regulations of fibroblast growth factor signaling during embryonic development.
Thisse B, Thisse C.
Dev Biol. 2005 Nov 15;287(2):390-402. Epub 2005 Oct 10. (REVIEW)
PMID 16216232
 
TONDU (TDU), a novel human protein related to the product of vestigial (vg) gene of Drosophila melanogaster interacts with vertebrate TEF factors and substitutes for Vg function in wing formation.
Vaudin P, Delanoue R, Davidson I, Silber J, Zider A.
Development. 1999 Nov;126(21):4807-16.
PMID 10518497
 

Citation

This paper should be referenced as such :
Nord, KH
Myxoinflammatory fibroblastic sarcoma (MIFS) with t(1;10)(p22;q24)
Atlas Genet Cytogenet Oncol Haematol. 2012;16(7):509-513.
Free journal version : [ pdf ]   [ DOI ]
On line version : http://AtlasGeneticsOncology.org/Tumors/t0110p22q24MyoInfFibSID6369.html


Other genes implicated (Data extracted from papers in the Atlas) [ 1 ]

Genes TGFBR3

Translocations implicated (Data extracted from papers in the Atlas)

 t(1;10)(p22;q24) FGF8

External links

Mitelman database t(1;10)(p22;q24) FGF8 [Case List]    t(1;10)(p22;q24) FGF8 [Association List] Mitelman database (CGAP - NCBI)
arrayMap Topo ( C47,C49) arrayMap ((UZH-SIB Zurich)   [auto + random 100 samples .. if exist ]   [tabulated segments]
 
 
Disease databaseSoft Tissues: Myxoinflammatory fibroblastic sarcoma with t(1;10)(p22;q24) FGF8/
REVIEW articlesautomatic search in PubMed
Last year articlesautomatic search in PubMed


© Atlas of Genetics and Cytogenetics in Oncology and Haematology
indexed on : Fri Jan 27 12:49:12 CET 2017


Home   Genes   Leukemias   Solid Tumours   Cancer-Prone   Deep Insight   Case Reports   Journals  Portal   Teaching   

For comments and suggestions or contributions, please contact us

jlhuret@AtlasGeneticsOncology.org.