NONO (non-POU domain containing, octamer-binding)

1999-01-01 Jean-Loup Huret Affiliation

Genetics, Dept Medical Information, University of Poitiers, CHU Poitiers Hospital, F-86021 Poitiers, France

Identity

HGNC

NONO

LOCATION

Xq13.1

LOCUSID

4841

FUSION GENES

Show Gene Fusions

DNA/RNA

Transcription

alternative splicing; 2.4 mRNA complete cds; coding sequence: CDS 129 ... 1550

Proteins

Description

the protein encoded by NONO is called p54nrb (nuclear RNA binbing); 471 amino acids; 54 kDa; N-term glutamine/histidine rich region, tandem RNA binding domains (amino acids 75 to 228), helix-turn-helix implicated in DNA binding, and a proline rich region in C-term; N-term and C-term allow protein-protein interactions.

Expression

wide

Function

binds independantly to DNA and RNA; forms a heterodimer with PSF, a protein sharing vast homologies; both form complexes with DNA topoisomerase I, which renders this enzyme much more active; NONO also enhances the binding of several DNA-binding proteins (but not all).

Homology

with PSF and other proteins with a DBHS domain (Drosophila behaviour, human splicing) which includes the tandem RNA binding domains.

Implicated in

Entity name

inv(X)(p11.2q12) in renal cell carcinoma -->NONO/TFE3

Disease

only one case of papillary renal cell carcinoma

Hybrid gene

5 NONO- 3 TFE3

Fusion protein

N-term NONO and most of it except the C-term proline rich sequence fused to the DNA binding domains of TFE3 (excluding the acidic transcriptional activation domain, including the C-term helix-loop-helix, and the leucine zipper); the reciprocal transcript is found

Article Bibliography

Pubmed ID	Last Year	Title	Authors
9393982	1997	Fusion of splicing factor genes PSF and NonO (p54nrb) to the TFE3 gene in papillary renal cell carcinoma.	Clark J et al
8371983	1993	Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6.	Dong B et al
9756848	1998	The RNA-splicing factor PSF/p54 controls DNA-topoisomerase I activity by a direct interaction.	Straub T et al
8355702	1993	NonO, a non-POU-domain-containing, octamer-binding protein, is the mammalian homolog of Drosophila nonAdiss.	Yang YS et al
9171077	1997	NonO enhances the association of many DNA-binding proteins to their targets.	Yang YS et al

Other Information

Locus ID:

NCBI: 4841
MIM: 300084
HGNC: 7871
Ensembl: ENSG00000147140

Variants:

dbSNP: 4841
ClinVar: 4841
TCGA: ENSG00000147140
COSMIC: NONO

RNA/Proteins

Gene ID	Transcript ID	Uniprot
ENSG00000147140	ENST00000276079	Q15233
ENSG00000147140	ENST00000276079	A0A0S2Z4Z9
ENSG00000147140	ENST00000373841	Q15233
ENSG00000147140	ENST00000373841	A0A0S2Z4Z9
ENSG00000147140	ENST00000373856	Q15233
ENSG00000147140	ENST00000373856	A0A0S2Z4Z9
ENSG00000147140	ENST00000413858	C9JYS8
ENSG00000147140	ENST00000418921	H7C367
ENSG00000147140	ENST00000420903	C9J4X2
ENSG00000147140	ENST00000450092	C9JJ13
ENSG00000147140	ENST00000454976	C9IZL7
ENSG00000147140	ENST00000535149	Q15233

Expression (GTEx)

100

150

200

250

Adipose - Subcutaneous

Adipose - Visceral

Adrenal Gland

Artery - Aorta

Artery - Tibial

Bladder

Brain - Amygdala

Brain - Anterior cingulate cortex

Brain - Caudate

Brain - Cerebellar Hemisphere

Brain - Cerebellum

Brain - Cortex

Brain - Frontal Cortex

Brain - Hippocampus

Brain - Hypothalamus

Brain - Nucleus accumbens

Brain - Putamen

Brain - Spinal cord

Brain - Substantia nigra

Breast - Mammary Tissue

Cells - Cultured fibroblasts

Cells - EBV - transformed lymphocytes

Cervix - Ectocervix

Cervix - Endocervix

Colon - Sigmoid

Colon - Transverse

Esophagus - Gastroesophageal Junction

Esophagus - Mucosa

Esophagus - Muscularis

Fallopian Tube

Heart - Atrial Appendage

Heart - Left Ventricle

Kidney - Cortex

Kidney - Medulla

Liver

Lung

Minor Salivary Gland

Muscle - Skeletal

Nerve - Tibial

Ovary

Pancreas

Pituitary

Prostate

Skin - Not Sun Exposed

Skin - Sun Exposed

Small Intestine - Terminal Ileum

Spleen

Stomach

Testis

Thyroid

Uterus

Vagina

Whole Blood

Protein levels (Protein atlas)

Not detected

Low

Medium

High

References

Pubmed ID	Year	Title	Citations
38211652	2024	IKZF4/NONO-RAB11FIP3 axis promotes immune evasion in gastric cancer via facilitating PD-L1 endosome recycling.	0
38224452	2024	Nucleolar detention of NONO shields DNA double-strand breaks from aberrant transcripts.	1
38341127	2024	NONO promotes gallbladder cancer cell proliferation by enhancing oncogenic RNA splicing of DLG1 through interaction with IGF2BP3/RBM14.	1
38211652	2024	IKZF4/NONO-RAB11FIP3 axis promotes immune evasion in gastric cancer via facilitating PD-L1 endosome recycling.	0
38224452	2024	Nucleolar detention of NONO shields DNA double-strand breaks from aberrant transcripts.	1
38341127	2024	NONO promotes gallbladder cancer cell proliferation by enhancing oncogenic RNA splicing of DLG1 through interaction with IGF2BP3/RBM14.	1
36416237	2023	NONO enhances mRNA processing of super-enhancer-associated GATA2 and HAND2 genes in neuroblastoma.	8
36459792	2023	NONO regulates multiple cytokine production in sepsis via the ERK1/2 signaling pathway.	0
36653413	2023	A novel NONO variant that causes developmental delay and cardiac phenotypes.	1
37271897	2023	The Conserved LncRNA DIO3OS Restricts Hepatocellular Carcinoma Stemness by Interfering with NONO-Mediated Nuclear Export of ZEB1 mRNA.	7
37370134	2023	Non-POU Domain-Containing Octomer-Binding (NONO) protein expression and stability promotes the tumorigenicity and activation of Akt/MAPK/β-catenin pathways in human breast cancer cells.	3
36416237	2023	NONO enhances mRNA processing of super-enhancer-associated GATA2 and HAND2 genes in neuroblastoma.	8
36459792	2023	NONO regulates multiple cytokine production in sepsis via the ERK1/2 signaling pathway.	0
36653413	2023	A novel NONO variant that causes developmental delay and cardiac phenotypes.	1
37271897	2023	The Conserved LncRNA DIO3OS Restricts Hepatocellular Carcinoma Stemness by Interfering with NONO-Mediated Nuclear Export of ZEB1 mRNA.	7

Citation

Jean-Loup Huret

NONO (non-POU domain containing, octamer-binding)

Atlas Genet Cytogenet Oncol Haematol. 1999-01-01

Online version: http://atlasgeneticsoncology.org/gene/168/js/lib/cancer-prone-explorer/gene-fusions-explorer/