A new case of t(8;14)(q11;q32) in an acute lymphoblastic leukemia
2003-05-01 Benoit Quilichini, Helene Zattara, Franois Casalonga, Laure-Anne Bastide-Alliez, Catherine Curtillet, Chantal Fossat, Gérard Michel AffiliationCytogenetic Oncology Laboratory : CHU Timone 264, rue Saint-Pierre 13385 Marseille cedex 5, France ; Tel : +33-491387402 ï Fax : +33-491384663 e-mail : [email protected], [email protected]
Previous history
Preleukaemia
-
Malignant disease
-
Inborn condition
-
Clinics case report
Age
13 yrs
Sex
M
Liver
-
Spleen
-
Lymph nodes
-
Cns involv
-
Blood data
Wbc
21
Hb
: 9.1
Platelets
32
Blasts
79
Bone marrow
97
Cyto path
Cytology
neutrophils : 11 %; eosinophils : 0 %; basophils : 0%; lymphocytes : 9 %; monocytes : 1%
Immunophenotype
HLA DR+ (high), CD34+ (high), CD10+ (low), CD19+ (high), c mu -, CD79a+ (high). Blasts were myeloperoxydase negative.
Precise diagnosis
Diagnosis of precursor B-cell acute lymphoblastic leukemia (WHO classification), LAL-B II (EGIL classification).
Survival data
Date diagnosis
04-2003
Complete remission
+
Karyotype
Sample
Blood and bone marrow.
Culture time
Overnight unstimulated culture
Banding
R-BANDING
Results
45, X, - Y, t(8;14)(q11;q32) [ 16 ] / 46, XY [ 4 ]
Mol cytogenet technics
2- FISH was performed using a chimeric X/Y direct labelled coktail probe (Qbiogene) according to the manufacturer s instructions. FISH confirmed the loss of chromosome Y in 90% on interphase cells (data not shown).
Other molec studies
Results
Images
Figure 1: Bone marrow (MGG-stained ) x 50: Increased cellularity, no megakaryocyte. Blasts : 97 % ; Neutrophils : 3%.
Figure 2: Karyotype ( R-bands) : 45, X, -Y, t(8;14)(q11;q32).
Figure 3: Fluorescence in situ hybridization using WCP 8 probe ( Spectrum Orange) and LSI-IgH locus probe ( FITC): partial karyotype results ( R-banding and FISH ).
Comments section
Comments
The breakpoint at 8q11 has not yet been cloned.
Article Bibliography
| Pubmed ID | Last Year | Title | Authors |
|---|---|---|---|
| 7564526 | 1995 | Proposals for the immunological classification of acute leukemias. European Group for the Immunological Characterization of Leukemias (EGIL). | Bene MC et al |
| 10577857 | 1999 | World Health Organization classification of neoplastic diseases of the hematopoietic and lymphoid tissues: report of the Clinical Advisory Committee meeting-Airlie House, Virginia, November 1997. | Harris NL et al |
| 10673739 | 2000 | Acute lymphoblastic leukemia with an unusual t(8;14)(q11.2;q32): a Pediatric Oncology Group Study. | Kaleem Z et al |
| 12581891 | 2003 | Acute lymphoblastic leukemia characterized by t(8;14)(q11.2;q32). | Moore S et al |
Citation
Benoit Quilichini, Helene Zattara, Franois Casalonga, Laure-Anne Bastide-Alliez, Catherine Curtillet, Chantal Fossat, Gérard Michel
A new case of t(8;14)(q11;q32) in an acute lymphoblastic leukemia
Atlas Genet Cytogenet Oncol Haematol. 2003-05-01
Online version: http://atlasgeneticsoncology.org/case-report/208805/a-new-case-of-t(8
