ins(9;4)(q33;q12q25) CDK5RAP2/PDGFRA

2010-03-01   Jean-Loup Huret 

1.Genetics, Dept Medical Information, University of Poitiers, CHU Poitiers Hospital, F-86021 Poitiers, France

Clinics and Pathology

Disease

Chronic eosinophilic leukemia

Epidemiology

One case to date, a 71-year-old female patient with chronic eosinophilic leukemia in accelerated phase (Walz et al., 2006)..

Prognosis

Remission was obtained with imatinib, but the patient relapsed with imatinib-resistant acute myeloid leukemia that was characterized by a normal karyotype, absence of detectable CDK5RAP2-PDGFRA mRNA, and a newly acquired G12D NRAS mutation.

Genes Involved and Proteins

Gene name
PDGFRA (platelet-derived growth factor receptor, alpha polypeptide)
Location
4q12
Protein description
Receptor tyrosine kinase. Gain-of-function mutations of PDGFRA are implicated in a subset of gastrointestinal stromal tumors (Heinrich et al., 2003). PDGFRA has also been involved in translocations, making hybrid genes with STRN (2p22), FIP1L1 (4q12), KIF5B (10p11), ETV6 (12p13) and BCR (22q11).
Gene name
CDK5RAP2 (CDK5 regulatory subunit associated protein 2)
Location
9q33.2
Protein description
Centrosomal protein; regulates CDK5; binds EB1. The CDK5RAP2-EB1 complex stimulates microtubule assembly (Fong et al., 2009); critical for centrosomal localization of dynein throughout the cell cycle (Lee and Rhee, 2010). CDK5RAP2-knockdown cells have increased resistance to paclitaxel and doxorubicin (Zhang et al., 2009). Homozygous mutations in CDK5RAP2 can cause microcephaly (Bond et al., 2005).

Result of the Chromosomal Anomaly

Description

In-frame fusion between exon 13 of the CDK5RAP2, a 40 bp insert from an inverted sequence of PDGFRA intron 9, and a truncated PDGFRA exon 12. No reciprocal PDGFRA-CDK5RAP2 transcript.N-term CDK5RAP2 - C-term PDGFRA; 1003 amino acids; contains 494 amino acids, including several potential dimerization domains, of CDK5RAP2 and 509 amino acids from PDGFRA tyrosine kinase domains.

Oncogenesis

Constitutive tyrosine kinase activity is likely.

Highly cited references

Pubmed IDYearTitleCitations
168456592006Transient response to imatinib in a chronic eosinophilic leukemia associated with ins(9;4)(q33;q12q25) and a CDK5RAP2-PDGFRA fusion gene.14

Bibliography

Pubmed IDLast YearTitleAuthors

Summary

Fusion gene

CDK5RAP2/PDGFRA CDK5RAP2 (9q33.2) PDGFRA (4q12) M ins(9;4)(q33;q12q25)|CDK5RAP2/PDGFRA CDK5RAP2 (9q33.2) PDGFRA (4q12) TIC

Citation

Jean-Loup Huret

ins(9;4)(q33;q12q25) CDK5RAP2/PDGFRA

Atlas Genet Cytogenet Oncol Haematol. 2010-03-01

Online version: http://atlasgeneticsoncology.org/haematological/1450/js/lib/jquery-3.5.1.min.js