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RHOBTB2 (Rho-related BTB domain containing 2)

Written2008-12Kristina Schenková, Francisco Rivero
Centre for Biomedical Research, The Hull York Medical School, University of Hull, Cottingham Road, Hull HU6 7RX, UK
Updated2016-02Kristina Schenková, Shuo Cai, Francisco Rivero
Centre for Cardiovascular and Metabolic Research, The Hull York Medical School, University of Hull, Cottingham Road, Hull HU6 7RX, UK; kristina.schenkova@hyms.ac.uk, CaiS@cardiff.ac.uk, francisco.rivero@hyms.ac.uk

Abstract RhoBTB2 is one of the three members of the RhoBTB family. All RhoBTB proteins are characterized by a GTPase domain followed by a proline-rich region, a tandem of two BTB domains and a C-terminal putative RING finger domain. RHOBTB2 is a putative tumour suppressor gene. Expression of RHOBTB2 has been found decreased in breast, lung and bladder tumors, head and neck squamous cell carcinoma and osteosarcoma. Decreased expression is often the result of promoter methylation. Mutations are uncommon. The role of RhoBTB2 in tumorigenesis is unknown but may be related to its function as a component of Cullin 3-dependent ubiquitin ligase complexes regulating the cell cycle and apoptosis.

Keywords tumor suppressor, ubiquitin ligase, cullin 3

(Note : for Links provided by Atlas : click)

Identity

Alias_namesRho-related BTB domain containing 2
Alias_symbol (synonym)KIAA0717
DBC2
Other aliasDBC2 (Deleted in breast cancer 2 gene protein)
HGNC (Hugo) RHOBTB2
LocusID (NCBI) 23221
Atlas_Id 42109
Location 8p21.3  [Link to chromosome band 8p21]
Location_base_pair Starts at 22987417 and ends at 23020197 bp from pter ( according to hg19-Feb_2009)  [Mapping RHOBTB2.png]
Local_order The RHOBTB2 locus is placed in a region of chromosome 8 (8p22-8p21.3) that exhibits frequent allelic imbalance and contains several tumor related genes.
Fusion genes
(updated 2016)
RHOBTB2 (8p21.3) / PNOC (8p21.1)RHOBTB2 (8p21.3) / STK40 (1p34.3)TSC22D3 (Xq22.3) / RHOBTB2 (8p21.3)

DNA/RNA

 
  Gene structure of RHOBTB2. Boxes represent exons. The coding region is represented in blue.
Description The RHOBTB2 gene spans over 20 Kbp genomic DNA and consists of 10 exons, 9 coding exons and one exon in the 5'UTR (Figure 1). The coding sequence of RHOBTB2 is 2184 nucleotides long. The promoter region of RHOBTB2 has CpG islands.
Transcription There is no evidence of alternatively spliced transcripts.

Protein

Note RhoBTB2 is one of the three members of the RhoBTB family in vertebrates. The RhoBTB family was identified during the study of the genes encoding Rho-related proteins in the lower eukaryote Dictyostelium discoideum (Rivero et al., 2002). All three RhoBTB proteins may be implicated in tumorigenesis.
 
  Architecture of RhoBTB proteins. The figure shows the three human (Hs) RhoBTB subfamily members as well as the Drosophila (Dm) and Dictyostelium (Dd) orthologues. The simplified phylogenetic tree on the left illustrates the relationship among the proteins (overall percentage similarity between branches). The different domains are indicated with colours.
Description RhoBTB2 is 727 amino acids long. All RhoBTB proteins share the same domain architecture: a GTPase domain is followed by a proline-rich region, a tandem of two BTB domains and a C-terminal region (Figure 2)
The GTPase domain is Rho-related and contains a Rho insert that is longer than usual, two insertions and one deletion, as well as a few deviations from the GTPase consensus of most Rho GTPases. Chang et al. (2006) reported the inability of this domain to bind GTP. However the construct used in that study lacked one of the GTPase motifs, G5. Subsequent work with the full-length protein and a more complete GTPase domain showed that this domain is actually able to bind GTP (Manjarrez et al., 2014).
The proline-rich region links the GTPase to the first BTB domain. This region could act as a SH3 domain-binding site.
The BTB domain (broad complex, tramtract and bric-a-brac) is an evolutionary conserved protein-protein interaction domain that participates in homomeric and heteromeric associations with other BTB domains. The BTB domain was also identified as a component of multimeric cullin3-dependent ubiquitin ligase complexes. The first BTB domain is bipartite, being interrupted by an insertion of unknown function. The BTB domains of RhoBTB allow the formation of homodimers and of heterodimers with other proteins of the RhoBTB family (Berthold et al., 2008).
The C-terminus is a region conserved in all members of the RhoBTB subfamily. It predictably folds as 4 consecutive alpha-helices and one beta-strand and may constitute a RING finger domain (Manjarrez et al., 2014). Many RING finger domains function as ubiquitin ligases. RhoBTB2 does not bear a CAAX motif that is typical for classical Rho GTPases and serves for localization of the protein to membranes.
Expression RHOBTB2 is weakly expressed, with relatively higher levels in neural and cardiac tissues. It is also expressed in fetal tissues (Ramos et al., 2002; Nagase et al., 1998). During mouse embryogenesis high and specific expression has been observed in the central and peripheral nervous system and comparatively weaker in the gut, but the mRNA becomes undetectable at embryonic day 18.5 (St-Pierre et al., 2004). One study has addressed the expression of RHOBTB2 during mammogenesis in the mouse and found that transcripts are expressed at low but constant levels. Attempts to study the spatial pattern of expression in the mammary gland using in situ hybridization were inconclusive because of undetectable mRNA levels (St-Pierre et al., 2004).
RhoBTB2 levels increase upon initiation of prophase and decrease at telophase. RhoBTB2 levels also increase during drug-induced apoptosis. Both effects depend on the E2F1 transcription factor (Freeman et al., 2007).
RHOBTB2 is a TP53 candidate target gene (Garritano et al., 2013).
Expression of RHOBTB2 has been found decreased in breast, lung, bladder and stomach cancers and in osteosarcomas (Hamaguchi et al., 2002; Knowles et al., 2005; Cho et al., 2008; Shi et al., 2008; Dong et al., 2012; Han et al., 2013; Jin et al., 2013) as well as in cell lines derived from breast, lung and bladder tumors and head and neck squamous cell carcinomas (Hamaguchi et al., 2002; Knowles et al., 2005; McKinnon et al., 2008) Loss of RHOBTB2 expression has been found to correlate with promoter methylation in breast and bladder cancers (Shi et al., 2008; Mehri Hajikhan et al., 2012; Han et al., 2013).
Localisation The localisation of the endogenous RhoBTB2 protein has not been investigated extensively. In cells ectopically expressing RhoBTB2 the protein tends to form aggregates in the cytoplasm (Aspenström et al., 2004 ; Berthold et al., 2008). When expressed at moderate levels it displays a vesicular pattern, frequently in the proximity of microtubules (Chang et al., 2006 ; Berthold et al., 2008).
Function RHOBTB2 was initially described as a gene homozygously deleted in breast cancer samples and was proposed as a candidate tumor supressor gene (Hamaguchi et al., 2002). The mechanisms by which RhoBTB2 exerts this and other roles remain speculative. When expressed in cancer cell lines, RhoBTB2 inhibits proliferation, induces apoptosis and inhibits cell migration and invasion (Mao et al., 2011; Jin et al., 2013).
Following functions have been proposed for RhoBTB2:
1. RhoBTB2 as adaptor of cullin3-dependent ubiquitin ligases. The first BTB domain binds to the N-terminal region of Cullin 3, but not other cullins. RhoBTB2 is itself a substrate for the Cullin 3-based ubiquitin ligase complex (Wilkins et al., 2004). RhoBTB proteins appear to exist in an inactive state through an intramolecular interaction of the BTB domain region with the GTPase domain (Berthold et al., 2008). This model has been refined recently to show that the HSP90AA1 (Hsp90) chaperone machinery unlocks RhoBTB, enabling GTP binding and interaction with Cullin 3 and the COPS8 (COP9) signalosome. COP9 deneddylates Cullin 3 and stabilises the complex (Manjarrez et al. 2014).
RhoBTB2, like RhoBTB1 and RhoBTB3, interacts with LLRC41 (leucine rich repeat containing 41, MUF1). MUF1 is a nuclear protein and carries a BC-box that functions as a linker in multicomponent Cullin 5-dependent ubiquitin ligase complexes (Schenková et al., 2012). MUF1 may be a substrate for RhoBTB-Cullin 3 ubiquitin ligase complexes. The function of MUF1 is unknown, but it is suspected to be involved in the DNA damage response.
2. RhoBTB2, cell growth and apoptosis. Overexpression of RhoBTB2 in the breast cancer cell line T-47D (a cell line that lacks RHOBTB2 transcripts) effectively suppressed cell growth in vitro (Hamaguchi et al., 2002). Overexpression of RhoBTB2 in osteosarcoma cells significantly arrested cells at G1 and resulted in apoptosis (Jin et al., 2013). In the thyroid carcinoma cell line SW579 treatment with recombinant RhoBTB2 for 24 hours inhibited proliferation and provoked an increase of the apoptotic ratio through the mitochondrial apoptotic signalling pathway (Wang et al., 2015), but it's not clear how the exogenously added protein exerts those actions.
It was shown that overexpression of RhoBTB2 leads to a short-term increase in cell cycle progression and proliferation, but long-term expression has a negative effect on proliferation (Freeman et al., 2007). The growth arrest effect has been explained by the downregulation of CCND1 (cyclin D1). Cyclin D1 is upstream of cyclin E, and the overexpression of any of both prevented the growth arrest effect of RhoBTB2 (Yoshihara et al., 2007). The effect on cyclin D1 is probably post-transcriptional, but only partially dependent on proteasomal degradation (Collado et al., 2007). RHOBTB2 has been identified as a target of the E2F1 transcription factor. RhoBTB2 levels also increase during drug-induced apoptosis in an E2F1-dependent manner, and the downregulation of RHOBTB2 delays the onset of apoptosis (Freeman et al., 2007).
3. RhoBTB2 and chemokine expression. Downregulation of RhoBTB2 by RNA interference in primary lung epithelial cells causes a decrease in CXCL14 mRNA expression. The same effect was observed in keratinocytes and is apparently independent of Cullin 3-mediated protein degradation (McKinnon et al., 2008).
4. RhoBTB2 and vesicle transport. Knockdown of endogenous RhoBTB2 hindered the ER to Golgi apparatus transport of a VSVG-GFP reporter and resulted in the altered distribution of the fusion protein. Ectopic RhoBTB2 distributes in a vesicular pattern occasionally adjacent to microtubules and an intact microtubule network seems to be required for the mobility of RhoBTB2 (Chang el al., 2006).
5. RhoBTB2 and the actin filament system. RhoBTB2 displays only a moderate influence on the morphology and actin organisation of porcine aortic endothelial cells upon ectopic expression. It does not interact with the GTPase-binding domain of WASP, PAK1 or RTKN (Rhotekin), which are well-known effectors of many typical Rho GTPases (Aspenström et al., 2004).
Homology There are three RhoBTB proteins in vertebrates: RhoBTB1, RhoBTB2 and RhoBTB3 (Figure 2). RhoBTB2 is very similar to RhoBTB1, while RhoBTB3 displays very low similarity to these. Orthologues have been found in amoebae and in insects but they are absent in plants and fungi.

Mutations

Note Following table compiles mutations identified in RHOBTB2. Polymorphisms that could result in functional alterations are also included (marked with *). All mutations found in tumors are somatic. For mutations found in cell lines it has not been determined whether they are somatic or germinal.
Mutation

Effect

Tumour
Prom -238G>A*Altered expression?Breast
Prom -121C>TAltered expression?Breast
5'UTR +48G>AAltered expression?Breast
E5 C>T R275WUnknown effectStomach
E5 T>G Y284DAbolished binding to Cullin 3Lung (cell line)
E5 G>A D299NNo growth inhibition when re-expressedBreast
E5 G>C E349DUnknown effectBladder
E5 A>C D368AUnknown effectBreast (cell line)
E7 G>A G561S*Unknown effectBladder
E9 C>A P647TUnknown effectBreast
 
  Localisation of mutations found in the coding region of RHOBTB2 in tumors and cancer cell lines. Note that most missense mutations affect the first BTB domain of the protein and reside in exon 5.

Implicated in

Note
  
Entity Breast cancer
Note RHOBTB2 was found homozygously deleted in 3.5% of 200 breast tumors. A mutation analysis revealed two somatic missense mutations (E5 G>A D299N and E9 C>A P647T) and one missense mutation (E5 A>C D368A). In the same study expression of RHOBTB2 appeared extinguished in about 42% of 19 breast cancer cell lines (Hamaguchi et al., 2002).
A more extensive mutation analysis of 100 sporadic breast cancers revealed some polymorphisms as well as two somatic mutations in the promoter (-238G>A, -121C>T) and 5'UTR (+48G>A) of RHOBTB2. The analysis of 17 CC: TXT: familial breast tumours ID: 10062> negative for BRCA1/BRCA2mutations failed to reveal additional mutations in the coding region of RHOBTB2 (Ohadi et al., 2007).
No mutations were found in the promoter or exon 7 in 32 breast cancers of a Han Chinese population. This study revealed an intronic polymorphism common in this population; the variant IVS7 + 53C >G correlated with HER2 and p53 expression but not with age, tumor stage or estrogen or progesterone receptor expression (Fu et al., 2014.
Using semi-quantitative PCR RHOBTB2 mRNA was found absent (56% of 87 breast tumors vs. 9% of normal tissue) or significantly reduced. RHOBTB2 Promoter methylation was detected in over 33% of a large collection of breast tumor samples and was rare in normal tissue. Loss of RHOBTB2 expression correlated with promoter methylation. Moreover, RHOBTB2 promoter methylation associated with more advance tumor stages, p53 mutation and HER2-positive status (Han et al., 2013). A similar correlation between promoter methylation and RHOBTB2 downregulation has been reported in a sample of 50 paired breast cancer and normal tissues. In this study promoter methylation was found more frequently associated to progesterone receptor negative tumors (Tang et al., 2014). Significantly more frequent promoter methylation was also found in a collection of 50 breast tumor (34%) and blood samples (46%) compared to normal samples (20% or less) in another study (Mehri Hjikhan et al., 2012).
A frequent pattern of loss of RHOBTB2, CDH1 and TP53 together with gain of COX2 and MYC was found in a single cell in situ hybridization analysis of 13 samples simultaneously carrying ductal carcinoma in situ (DCIS) and invasive ductal carcinoma (IDC) of the breast. DCIS is the precursor lesion for invasive breast cancer. The study aimed at understanding the dynamics of genomic alterations in the progression from DCIS to IDC and included several oncogenes and tumor suppressor genes (Heselmayer-Haddad et al., 2012).
Oncogenesis RHOBTB2 was proposed as a candidate tumor suppressor gene based on the fact that its re-expression in T-47D (a breast cancer cell line that lacks RHOBTB2 transcripts) caused growth inhibition, whereas expression of the mutant D299N did not have the same effect. Neither the D299N nor a D368A mutant found in some tumors presented abolished binding to Cullin 3 (Hamaguchi et al., 2002). Overexpression of RhoBTB2 in T-47D inhibits cell proliferation and colony formation while promoting apoptosis, but was found not to influence the invasion and migration ability of the cells in a Transwell system (Mao et al., 2011).
The mutations found in the promoter and 5'UTR of RHOBTB2 in some breast tumors might affect regulation of gene expression (Ohadi et al., 2007). Lack of RHOBTB2 expression in T-47D is apparently due to RHOBTB2 promoter methylation (Han et al., 2013). Methylation of RHOBTB2 and other genes in peripheral blood is a potential epigenetic marker for predicting the risk of breast cancer development (Khakpour et al., 2015).
Ectopic expression of RHOBTB2 in two human metastatic breast cancer cell lines, MDA-MB-231 and MDA-MB-435, inhibits cell migration and invasiveness through a mechanism that involves upregulation of the metastasis suppressor BRMS1 and decreased phosphorylation of EZR (ezrin) and Akt2 (Ling et al., 2010). Ezrin is a cytoskeleton and signaling molecule that regulates cell adhesion, migration and invasion. Akt2 is a kinase involved in invasiveness of breast cancer cells and is able to phosphorylate ezrin (Freeman and Cress 2010)
  
  
Entity Lung cancer
Note A mutation analysis revealed a missense mutation (E5 T>G Y284D) in a lung tumour cell line. In the same study expression of RHOBTB2 appeared extinguished in about 50% of 14 lung cancer cell lines (Hamaguchi et al., 2002).
In an immunohistochemistry study of 172 tissue samples from different subtypes of lung adenocarcinomas frequent (70% of tumors) downregulation of RHOBTB2 was found that correlated with the degree of invasiveness (Dong et al, 2012).
Oncogenesis The Y284D mutant protein presents abolished binding to Cullin 3 and has consequently a longer half-life than the wild type protein. The mutation resides in the dimerisation interface of the first BTB domain and could prevent proper folding (Wilkins et al., 2004).
  
  
Entity Gastric cancer
Note In a study on primary gastric cancers loss of heterozygosity was found in 29% of 95 tumors. Sequence analysis identified several polymorphisms and one missense somatic mutation (E5 C>T R275W) of unknown effect (Cho et al., 2008).
  
  
Entity Bladder cancer
Note A loss of heterozygosity (LOH) and mutation analysis on 54 tumour samples and 32 cell lines of bladder cancer revealed LOH in the target region in 42% of informative tumours and 38% of cell lines. Sequence analysis revealed numerous polymorphisms and one missense somatic mutation (E5 G>C E349D) of unknown effect. One polymorphism (E7 G>A G561S) may have some functional effect. In addition, expression of RHOBTB2 was found reduced by 2 to 20-fold in 9 of 12 cell lines with predicted LOH in the region of interest (Knowles et al. 2005). Significantly higher RHOBTB2 promoter methylation correlated with decreased expression compared to normal tissue was reported in a study of 75 bladder cancer samples (Shi et al., 2008).
  
  
Entity Head and neck squamous cell carcinoma
Note Expression of RHOBTB2 was found reduced in four clonal keratinocyte cell lines derived from patients with HNSCC. This was accompanied by reduced expression of the chemokine CXCL14 (McKinnon et al., 2008).
Oncogenesis RhoBTB2 seems to be required for expression of the chemokine CXCL14 (McKinnon et al., 2008). CXCL14 controls leukocyte migration and angiogenesis and its expression is frequently lost in diverse epithelial tumours, including most HNSCCs.
  
  
Entity Osteosarcoma
Note Expression of RhoBTB2 was analysed in 121 osteosarcoma specimens and was found decreased compared to normal regions of the specimens. Expression correlated with the primary location and local recurrence of the tumor (Jin et al., 2013).
Oncogenesis RhoBTB2 may affect the cell cycle. Overexpression of RhoBTB2 in osteosarcoma cells significantly arrested cells at G1 and resulted in apoptosis (Jin et al., 2013).
  

Bibliography

The mutation of DBC2 in breast cancer patients from the Han ethnic group in Eastern China.
Fu G, Wang H, Wu H, Wang M.
Hematol Oncol Stem Cell Ther 2014; 7: 59-62
PMID 24485767
 
RhoBTB2 gene in breast cancer is silenced by promoter methylation.
Tang W, Wang C, Fu F, Chen Q.
Int J Mol Med 2014; 33: 722-728
PMID 24356943
 
Rho GTPases have diverse effects on the organization of the actin filament system.
Aspenströ P, Fransson A, Saras J.
Biochem J 2004? 377: 327-337.
PMID 14521508
 
Rho GTPases of the RhoBTB subfamily and tumorigenesis.
Berthold J, Schenková K, Rivero F.
Acta Pharmacol Sin 2008; 29: 285-295. (Review)
PMID 18298893
 
DBC2 is essential for transporting vesicular stomatitis virus glycoprotein.
Chang FK, Sato N, Kobayashi-Simorowski N, Yoshihara T, Meth JL, Hamaguchi M.
J Mol Biol 2006? 364: 302-308.
PMID 17023000
 
Genetic analysis of the DBC2 gene in gastric cancer.
Cho YG, Choi BJ, Kim CJ, Song JH, Zhang C, Nam SW, Lee JY, Park WS.
Acta Oncol 2008; 47: 366-371.
PMID 17906984
 
DBC2 resistance is achieved by enhancing 26S proteasome-mediated protein degradation.
Collado D, Yoshihara T, Hamaguchi M.
Biochem Biophys Res Commun 2007; 360: 600-603.
PMID 17617377
 
Distinct and overlapping roles for E2F family members in transcription, proliferation and apoptosis.
DeGregori J, Johnson DG.
Curr Mol Med 2006; 6: 739-748.
PMID 17100600
 
Loss of DBC2 expression is an early and progressive event in the development of lung adenocarcinoma.
Dong, W., Meng, L., Shen, HC. And Du, JJ.
Asian Pacif J Cancer Prev 2012; 13: 2021-2013
PMID 22901165
 
RhoBTB2 (DBC2) comes of age as a multifunctional tumor suppressor.
Freeman SN, Cress WD.
Cancer Biol Ther 2010; 10: 1123-1125.
PMID 20980811
 
More targets, more pathways and more clues for mutant p53.
Garritano S, Inga A, Gemignani F, Landi S.
Oncogenesis 2013; 2:e54.
PMID 23817466
 
Evaluation of methylation status in the 5'UTR promoter region of the DBC2 gene as a biomarker in sporadic breast cancer.
Hajikhan Mirzaei M, Noruzinia M, Karbassian H, Shafeghati Y, Keyhanee M, Bidmeshki-Pour A.
Cell J 2012; 14: 19-24
PMID 23626933
 
DBC2, a candidate for a tumor suppressor gene involved in breast cancer.
Hamaguchi M, Meth JL, von Klitzing C, Wei W, Esposito D,Rodgers L, Walsh T, Welcsh P, King M-C, Wigler MH.
Proc Natl Acad Sci USA 2002; 99: 13647-13652.
PMID 12370419
 
Decreased expression of the DBC2 gene and its clinicopathological significance in breast cancer: correlation with aberrant DNA methylation.
Han L, Hou L, Song J, Lin D, Wu L, Ge Y, Ma Z.
Biotechnol Lett 2013; 35: 1175-1181
PMID 23546941
 
Single-cell genetic analysis of ductal carcinoma in situ and invasive breast cancer reveals enormous tumor heterogeneity yet conserved genomic imbalances and gain of MYC during progression.
Heselmeyer-Haddad K, Berroa Garcia LY, Bradley A, Ortiz-MelendezC , Lee W-J, Christensen R, Prindiville SA, Calzone KA, Soballe PW, Hu Y, Chowdhury SA, Schwartz, R, Schäffer AA, Ried T.
Am J Pathol 2012; 181: 1807-1822
PMID 23062488
 
Downregulated RhoBTB2 expression contributes to poor outcome in osteosarcoma patients.
Jin Z, Han Y, Han X.
Cancer Biother Radiopharm 2013; 28: 709-716.
PMID 23777252
 
DNA methylation as a promising landscape: a simple blood test for breast cancer prediction.
Khakpour G, Pooladi A, Izad P, Noruzinia M, Tavakkoly Bazzaz J.
Tumour Biol 2015; 36: 4905-4912
PMID 26076810
 
Mutation analysis of the 8p candidate tumour suppressor genes DBC2 (RHOBTB2) and LZTS1 in bladder cancer.
Knowles MA, Aveyard JS, Taylor CF, Harnden P, Bass S.
Cancer Lett 2005; 225: 121-130.
PMID 15922864
 
Ectopic expression of RhoBTB2 inhibits migration and invasion of human breast cancer cells.
Ling L, Lu C, Zhou G, Wang S.
Cancer Biol Ther 2010; 10: 1115-1122.
PMID 20930524
 
Hsp90-dependent assembly of the DBC2/RhoBTB2-Cullin3 E3-ligase complex.
Manjarrez JR, Sun L, Prince T, Matts RL.
PLoS One 2014; 9: e90054
PMID 24608665
 
RhoBTB2 (DBC2) functions as tumor suppressor via inhibiting proliferation, preventing colony formation and inducing apoptosis in breast cancer cells.
Mao H, Zhang L, Yang Y, Sun J, Deng B, Feng J, Shao Q, Feng A, Song B, Qu X,
Gene 2011; 486: 74-80.
PMID 21801820
 
The atypical Rho GTPase RhoBTB2 is required for expression of the chemokine CXCL14 in normal and cancerous epithelial cells.
McKinnon CM, Lygoe KA, Skelton L, Mitter R, Mellor H.
Oncogene 2008; 27: 6856-6865.
PMID 18762809
 
Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.
Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O.
DNA Res 1998; 5: 355-364.
PMID 10048485
 
Mutation analysis of the DBC2 gene in sporadic and familial breast cancer.
Ohadi M, Totonchi M, Maguire P, Lindblom A, Habibi R, Alavi BA, Keyhani E, Najmabadi H.
Acta Oncol 2007; 46: 770-772.
PMID 17653899
 
Genomic organization and expression profile of the small GTPases of the RhoBTB family in human and mouse.
Ramos S, Khademi F, Somesh BP, Rivero F.
Gene 2002? 298: 147-157.
PMID 12426103
 
The Dictyostelium discoideum family of Rho-related proteins.
Rivero F, Dislich H, Glöckner G, Noegel AA.
Nucleic Acids Res 2001; 29: 1068-1079.
PMID 11222756
 
MUF1/Leucine-Rich Repeat Containing 41 (LRRC41), a substrate of RhoBTB-dependent cullin 3 ubiquitin ligase complexes, is a predominantly nuclear dimeric protein.
Schenková K, Lutz J, Kopp M, Ramos S, Rivero F.
J Mol Biol 2012; 422: 659-673.
PMID 22709582
 
DBC2 gene is silenced by promoter methylation in bladder cancer.
Shi Y, Chen JY, Yang J, Li B, Chen ZH, Xiao CG.
Urol Oncol 2008; 26: 465-469.
PMID 18640857
 
DBC2 significantly influences cell-cycle, apoptosis, cytoskeleton and membrane-trafficking pathways.
Siripurapu V, Meth J, Kobayashi N, Hamaguchi M.
J Mol Biol 2005; 346: 83-89.
PMID 15663929
 
High expression during neurogenesis but not mammogenesis of a murine homologue of the deleted in breast cancer2/Rhobtb2 tumor suppressor.
St-Pierre B, Jiang Z, Egan SE, Zacksenhaus E.
Gene Expr Patterns 2004; 5: 245-251.
PMID 15567721
 
RhoBTB2 (DBC2) functions as a multifunctional tumor suppressor in thyroid cancer cells via mitochondrial apoptotic pathway.
Wang C-J, Yand D, Luo Y-W.
Int J Clin Exp Med 2015; 8: 5954-5958
PMID 23546941
 
RhoBTB2 is a substrate of the mammalian Cul3 ubiquitin ligase complex.
Wilkins A, Ping Q, Carpenter CL.
Genes Dev 2004; 18: 856-861.
PMID 15107402
 
Cyclin D1 downregulation is essential for DBC2's tumor suppressor function.
Yoshihara T, Collado D, Hamaguchi M.
Biochem Biophys Res Commun 2007; 358: 1076-1079.
PMID 17517369
 

Citation

This paper should be referenced as such :
Schenková K, Cai S, Rivero F
RHOBTB2 (Rho-related BTB domain containing 2);
Atlas Genet Cytogenet Oncol Haematol. in press
On line version : http://AtlasGeneticsOncology.org/Genes/RHOBTB2ID42109ch8p21.html
History of this paper:
Schenkova, K ; Rivero, F. RHOBTB2 (Rho-related BTB domain containing 2). Atlas Genet Cytogenet Oncol Haematol. 2009;13(11):868-871.
http://documents.irevues.inist.fr/bitstream/handle/2042/44618/12-2008-RHOBTB2ID42109ch8p21.pdf


External links

Nomenclature
HGNC (Hugo)RHOBTB2   18756
Cards
AtlasRHOBTB2ID42109ch8p21
Entrez_Gene (NCBI)RHOBTB2  23221  Rho related BTB domain containing 2
AliasesDBC2
GeneCards (Weizmann)RHOBTB2
Ensembl hg19 (Hinxton)ENSG00000008853 [Gene_View]
Ensembl hg38 (Hinxton)ENSG00000008853 [Gene_View]  chr8:22987417-23020197 [Contig_View]  RHOBTB2 [Vega]
ICGC DataPortalENSG00000008853
TCGA cBioPortalRHOBTB2
AceView (NCBI)RHOBTB2
Genatlas (Paris)RHOBTB2
WikiGenes23221
SOURCE (Princeton)RHOBTB2
Genetics Home Reference (NIH)RHOBTB2
Genomic and cartography
GoldenPath hg38 (UCSC)RHOBTB2  -     chr8:22987417-23020197 +  8p21.3   [Description]    (hg38-Dec_2013)
GoldenPath hg19 (UCSC)RHOBTB2  -     8p21.3   [Description]    (hg19-Feb_2009)
EnsemblRHOBTB2 - 8p21.3 [CytoView hg19]  RHOBTB2 - 8p21.3 [CytoView hg38]
Mapping of homologs : NCBIRHOBTB2 [Mapview hg19]  RHOBTB2 [Mapview hg38]
OMIM607352   
Gene and transcription
Genbank (Entrez)AA641575 AB018260 AK092617 AK098744 AK292863
RefSeq transcript (Entrez)NM_001160036 NM_001160037 NM_015178
RefSeq genomic (Entrez)
Consensus coding sequences : CCDS (NCBI)RHOBTB2
Cluster EST : UnigeneHs.372688 [ NCBI ]
CGAP (NCI)Hs.372688
Alternative Splicing GalleryENSG00000008853
Gene ExpressionRHOBTB2 [ NCBI-GEO ]   RHOBTB2 [ EBI - ARRAY_EXPRESS ]   RHOBTB2 [ SEEK ]   RHOBTB2 [ MEM ]
Gene Expression Viewer (FireBrowse)RHOBTB2 [ Firebrowse - Broad ]
SOURCE (Princeton)Expression in : [Datasets]   [Normal Tissue Atlas]  [carcinoma Classsification]  [NCI60]
GenevisibleExpression in : [tissues]  [cell-lines]  [cancer]  [perturbations]  
BioGPS (Tissue expression)23221
GTEX Portal (Tissue expression)RHOBTB2
Protein : pattern, domain, 3D structure
UniProt/SwissProtQ9BYZ6   [function]  [subcellular_location]  [family_and_domains]  [pathology_and_biotech]  [ptm_processing]  [expression]  [interaction]
NextProtQ9BYZ6  [Sequence]  [Exons]  [Medical]  [Publications]
With graphics : InterProQ9BYZ6
Splice isoforms : SwissVarQ9BYZ6
PhosPhoSitePlusQ9BYZ6
Domaine pattern : Prosite (Expaxy)BTB (PS50097)    RHO (PS51420)   
Domains : Interpro (EBI)BTB/POZ_dom    P-loop_NTPase    SKP1/BTB/POZ    Small_GTPase   
Domain families : Pfam (Sanger)BTB (PF00651)    Ras (PF00071)   
Domain families : Pfam (NCBI)pfam00651    pfam00071   
Domain families : Smart (EMBL)BTB (SM00225)  
Conserved Domain (NCBI)RHOBTB2
DMDM Disease mutations23221
Blocks (Seattle)RHOBTB2
SuperfamilyQ9BYZ6
Human Protein AtlasENSG00000008853
Peptide AtlasQ9BYZ6
HPRD06300
IPIIPI00171950   IPI00816323   IPI00794883   IPI00930055   IPI01014308   
Protein Interaction databases
DIP (DOE-UCLA)Q9BYZ6
IntAct (EBI)Q9BYZ6
FunCoupENSG00000008853
BioGRIDRHOBTB2
STRING (EMBL)RHOBTB2
ZODIACRHOBTB2
Ontologies - Pathways
QuickGOQ9BYZ6
Ontology : AmiGOGTPase activity  protein binding  GTP binding  cytosol  plasma membrane  small GTPase mediated signal transduction  regulation of small GTPase mediated signal transduction  
Ontology : EGO-EBIGTPase activity  protein binding  GTP binding  cytosol  plasma membrane  small GTPase mediated signal transduction  regulation of small GTPase mediated signal transduction  
Pathways : KEGGUbiquitin mediated proteolysis   
REACTOMEQ9BYZ6 [protein]
REACTOME PathwaysR-HSA-194840 [pathway]   
NDEx NetworkRHOBTB2
Atlas of Cancer Signalling NetworkRHOBTB2
Wikipedia pathwaysRHOBTB2
Orthology - Evolution
OrthoDB23221
GeneTree (enSembl)ENSG00000008853
Phylogenetic Trees/Animal Genes : TreeFamRHOBTB2
HOVERGENQ9BYZ6
HOGENOMQ9BYZ6
Homologs : HomoloGeneRHOBTB2
Homology/Alignments : Family Browser (UCSC)RHOBTB2
Gene fusions - Rearrangements
Fusion : MitelmanRHOBTB2/PNOC [8p21.3/8p21.1]  
Fusion: TCGARHOBTB2 8p21.3 PNOC 8p21.1 BRCA
Polymorphisms : SNP and Copy number variants
NCBI Variation ViewerRHOBTB2 [hg38]
dbSNP Single Nucleotide Polymorphism (NCBI)RHOBTB2
dbVarRHOBTB2
ClinVarRHOBTB2
1000_GenomesRHOBTB2 
Exome Variant ServerRHOBTB2
ExAC (Exome Aggregation Consortium)RHOBTB2 (select the gene name)
Genetic variants : HAPMAP23221
Genomic Variants (DGV)RHOBTB2 [DGVbeta]
DECIPHERRHOBTB2 [patients]   [syndromes]   [variants]   [genes]  
CONAN: Copy Number AnalysisRHOBTB2 
Mutations
ICGC Data PortalRHOBTB2 
TCGA Data PortalRHOBTB2 
Broad Tumor PortalRHOBTB2
OASIS PortalRHOBTB2 [ Somatic mutations - Copy number]
Somatic Mutations in Cancer : COSMICRHOBTB2  [overview]  [genome browser]  [tissue]  [distribution]  
Mutations and Diseases : HGMDRHOBTB2
LOVD (Leiden Open Variation Database)Whole genome datasets
LOVD (Leiden Open Variation Database)LOVD - Leiden Open Variation Database
LOVD (Leiden Open Variation Database)LOVD 3.0 shared installation
BioMutasearch RHOBTB2
DgiDB (Drug Gene Interaction Database)RHOBTB2
DoCM (Curated mutations)RHOBTB2 (select the gene name)
CIViC (Clinical Interpretations of Variants in Cancer)RHOBTB2 (select a term)
intoGenRHOBTB2
NCG5 (London)RHOBTB2
Cancer3DRHOBTB2(select the gene name)
Impact of mutations[PolyPhen2] [SIFT Human Coding SNP] [Buck Institute : MutDB] [Mutation Assessor] [Mutanalyser]
Diseases
OMIM607352   
Orphanet
MedgenRHOBTB2
Genetic Testing Registry RHOBTB2
NextProtQ9BYZ6 [Medical]
TSGene23221
GENETestsRHOBTB2
Target ValidationRHOBTB2
Huge Navigator RHOBTB2 [HugePedia]
snp3D : Map Gene to Disease23221
BioCentury BCIQRHOBTB2
ClinGenRHOBTB2
Clinical trials, drugs, therapy
Chemical/Protein Interactions : CTD23221
Chemical/Pharm GKB GenePA38678
Clinical trialRHOBTB2
Miscellaneous
canSAR (ICR)RHOBTB2 (select the gene name)
Probes
Litterature
PubMed40 Pubmed reference(s) in Entrez
GeneRIFsGene References Into Functions (Entrez)
CoreMineRHOBTB2
EVEXRHOBTB2
GoPubMedRHOBTB2
iHOPRHOBTB2
REVIEW articlesautomatic search in PubMed
Last year publicationsautomatic search in PubMed

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