MIR200B (microRNA 200b)

2015-08-01   Yaguang Xi , Hong Chang 

Mitchell Cancer Institute, University of South Alabama, USA. xi@health.southalabama.edu; hchang@health.southalabama.edu

Identity

HGNC
LOCATION
1p36.33
LOCUSID
ALIAS
MIRN200B,mir-200b

Abstract

Review on MIR200B, with data on RNA, and where it is implicated.

DNA/RNA

Atlas Image

Description

microRNA 200b located in chromosome 1 and microRNA 200b was transcribed with microRNA 200a and microRNA 429 as a ploycistronic transcript. The putative transcription start site locates about 4228 bp upstream of the precursor of microRNA 200b.

Transcription

MiR-200b precursor: 5-CCAGCUCGGGCAGCCGUGGCCAUCUUACUGGGCAGCAUUGGAUGGAGUCAGGUCUCUAAUACUGCCUGGUAAUGAUGACGGCGGAGCCCUGCACG-3
Mature miR-200b: 5-UAAUACUGCCUGGUAAUGAUGA-3

Pseudogene

No pseudogene was found.

Proteins

Note

microRNAs are not translated into proteins.

Implicated in

Entity name
Tumor cell proliferation
Note
Multiple roles of miR-200b in cell cycles regulation and tumor cell proliferation were reported in human cancers. MiR-200b could directly target RND3 which could induce expression of cyclin D1 thereby promoting S-phase entry (Xia, Li et al. 2010). MiR-200b could also regulate cell cycle by targeting GATA4 and its downstream protein cyclin D1 which could affect cell proliferation (Yao, Wei et al. 2013). In human TGFBR2-null colorectal cancers, miR-200b could stimulate tumor growth by targeting CDKN1B (p27/kip1). It was also found that miR-200b could promoter cell proliferation by targeting p27/kip1 (Fu, Liu et al. 2014).
Entity name
Tumor cells invasion and cancer metastasis
Note
Abnormal expressions of miR-200b were found in various cancers, including breast cancer, colon cancer, nasopharyngeal carcinoma, urothelial carcinoma and prostate cancer. MiR-200b could directly targeting SUZ12 and ROCK2 thus inhibit cholangiocarcinoma tumourigenesis and metastasis (Peng, Jiang et al. 2013). In gastric carcinoma, miR-200b was found to target ZEB2 and thereby repress tumor cell invasion and migration (Kurashige, Kamohara et al. 2012).
Entity name
Repression of epithelial-mesenchymal transition (EMT)
Note
EMT is an important pathological progression and studies revealed vital roles of miR-200b in EMT regulation. MiR-200b could inhibit TGFB1-induced epithelial-mesenchymal transition by targeting SMAD2 intestinal epithelial cells and SMAD2 could repress expression of vimentin, which indicated regulation role of miR-200b on vimentin (Chen, Xiao et al. 2013). Also, the role of miR-200b in EMT regulation was observed in EMT induced by transforming growth factor-beta1 in kidney proximal tubular cells by directly target the 3-UTR of fibronectin (Tang, Chen et al. 2013).
Entity name
Chemoresistance
Note
Chemoresistance is the main cause of tumor relapse. Ectopic expression of miR-200b could reduce the chemoresistance of cells by targeting BIM1 in human tongue cancer cells. In human lung adenocarcinoma cells, miR-200b could target E2F3 to sensitize tumor cells to chemotherapy agents.

Bibliography

Pubmed IDLast YearTitleAuthors
234927722013miR-200b inhibits TGF-β1-induced epithelial-mesenchymal transition and promotes growth of intestinal epithelial cells.Chen Y et al
241510812014MicroRNA-200b stimulates tumour growth in TGFBR2-null colorectal cancers by negatively regulating p27/kip1.Fu Y et al
223111192012MicroRNA-200b regulates cell proliferation, invasion, and migration by directly targeting ZEB2 in gastric carcinoma.Kurashige J et al
241693432013Direct targeting of SUZ12/ROCK2 by miR-200b/c inhibits cholangiocarcinoma tumourigenesis and metastasis.Peng F et al
234081682013MiRNA-200b represses transforming growth factor-β1-induced EMT and fibronectin expression in kidney proximal tubular cells.Tang O et al
246678642014TGF-β1 stimulates human Tenon's capsule fibroblast proliferation by miR-200b and its targeting of p27/kip1 and RND3.Tong J et al
206836432010MicroRNA-200b regulates cyclin D1 expression and promotes S-phase entry by targeting RND3 in HeLa cells.Xia W et al
235587082013miR-200b targets GATA-4 during cell growth and differentiation.Yao CX et al

Other Information

Locus ID:

NCBI: 406984
MIM: 612091
HGNC: 31579
Ensembl: ENSG00000207730
miRBase:

Variants:

dbSNP: 406984
ClinVar: 406984
TCGA: ENSG00000207730
COSMIC: MIR200B

RNA/Proteins

Expression (GTEx)

0
1
2

Pathways

PathwaySourceExternal ID
MicroRNAs in cancerKEGGhsa05206
MicroRNAs in cancerKEGGko05206

References

Pubmed IDYearTitleCitations
208327272010Loss of miR-200 inhibition of Suz12 leads to polycomb-mediated repression required for the formation and maintenance of cancer stem cells.171
205510522010Pancreatic cancers epigenetically silence SIP1 and hypomethylate and overexpress miR-200a/200b in association with elevated circulating miR-200a and miR-200b levels.113
217253692012MiR-200b and miR-15b regulate chemotherapy-induced epithelial-mesenchymal transition in human tongue cancer cells by targeting BMI1.102
210790002010miR-200 family and targets, ZEB1 and ZEB2, modulate uterine quiescence and contractility during pregnancy and labor.95
210814892011miR-200b targets Ets-1 and is down-regulated by hypoxia to induce angiogenic response of endothelial cells.91
205140232010miR-200bc/429 cluster targets PLCgamma1 and differentially regulates proliferation and EGF-driven invasion than miR-200a/141 in breast cancer.77
175850492007miR-200b mediates post-transcriptional repression of ZFHX1B.68
240375282014MiR-200 can repress breast cancer metastasis through ZEB1-independent but moesin-dependent pathways.59
198016812009The miR200 family of microRNAs regulates WAVE3-dependent cancer cell invasion.56
223111192012MicroRNA-200b regulates cell proliferation, invasion, and migration by directly targeting ZEB2 in gastric carcinoma.56

Citation

Yaguang Xi ; Hong Chang

MIR200B (microRNA 200b)

Atlas Genet Cytogenet Oncol Haematol. 2015-08-01

Online version: http://atlasgeneticsoncology.org/gene/51137/mir200b-(microrna-200b)