ATR (ataxia telangiectasia and Rad3 related)

2010-05-01 Mary E Gagou , Mark Meuth Affiliation

Institute for Cancer Studies, The University of Sheffield, Medical School, Beech Hill Road, Sheffield, S10 2RX, UK

Identity

HGNC

ATR

LOCATION

3q23

LOCUSID

545

ALIAS

FCTCS,FRP1,MEC1,SCKL,SCKL1

FUSION GENES

Show Gene Fusions

DNA/RNA

Note

The first human ATR cDNA full-length clone (originally named FRP1, FRAP-related protein 1) was isolated from a Jurkat T-cell cDNA library and identified by its significant homology to other members of the phosphatidylinositol kinase-related kinase (PIKK) family. Evidence for the existence of two alternative ATR transcripts, with differential tissue expression, in the non-catalytic domain has been reported, by using RT-PCR. Transcript variants utilizing alternative polyadenylation signals are also exist. ATR gene has recently been annotated in the Ensembl database.

Intron/Exon structure of the ATR gene (ENSG00000175054). There are two transcript isoforms of this gene, with 47 and 46 exons, respectively, spanning to an area of 129.59 kb. Exons are illustrated with vertical lines and boxes (A, B). Diagram in panel B represents only the exons structure. Direction of transcription is shown by an arrow. The 6^th exon that is missing from ATR isoform 2 is indicated with an asterisk (A) or with blue colour box (B). Untranslated regions are in light pink, while coding regions are in deep pink. Start of translation (ATG) and stop codon (TGA) are also indicated.

Description

47 exons spanning to 129.59 kb.

Transcription

Two isoforms: isoform ATR-201 (ENST00000350721) (8248 bp) includes all 47 exons, while isoform ATR-202 (ENST00000383101) (8056 bp) does not include exon 6, deleting 192 nt (64 codons) from the mRNA. The translation start site is in exon 1.

Proteins

Note

ATR and ATM function in an overlapping, but non-redundant fashion, phosphorylating many of the same substrates. However, and in contrast to ATM, ATRs function is essential for cell viability. ATR-deficiency at the organismal level affects normal development, tissue homeostasis, and ageing.

Description

Isoform ATR-201 (ENSP00000343741): 2644 amino acids (predicted MW 301365.74 Da).
Isoform ATR-202 (ENSP00000372581): 2580 aa (predicted MW 294218.33 Da).
Biochemical studies of ATR protein do not distinguish between the two different isoforms.
ATR protein contains a PI3/4 kinase catalytic domain, 1 FAT domain, 1 FATC domain, 1 UME domain, and 2 HEAT repeats.

Expression

Isoform 1 has ubiquitous expression with highest levels in testis. Isoform 2 has more specific expression (has found in pancreas, liver and placenta while is not detected in heart, testis and ovary).
Forced expression of ATR inhibits MyoD function, leading to loss of differentiation, as well as induces cell-cycle abnormalities (increased aneuploidy and elimination of IR-induced G1 arrest). Limited expression of ATR or overexpression of kinase dead forms of this protein increases cell sensitivity to a variety of DNA damage agents and replication inhibitors, such as ionizing radiation (IR), cis-platinum, hydroxy urea (HU), methylmethanesulfonate (MMS) and ultra violet (UV) irradiation, leading to significant losses in checkpoint control and cell viability. Loss of ATR results in DNA fragile site expression, a specific type of genomic instability.

Localisation

In the nucleus, where it is recruited to chromatin during S-phase and redistributes to distinct foci upon DNA damage, stalling of replication forks with replication inhibitors or hypoxia. ATR has also been found in PML (promyetocytic leukaemia protein) nuclear bodies of some types of cells.
Kinase dead forms of ATR do not relocalize in response to IR and block nuclear translocation of RPA complex in a cell cycle-dependent manner.

Function

ATR essential maintains genome integrity by serving multiple roles in the cellular response to DNA damage and endogenous replication stress. It signals to regulate the firing of replication origins, the repair of damaged replication forks and to prevent the premature mitotic entry. Moreover, it critically functions directly at the sites of stalled forks by stabilizing components of the replisome to ensure completion of replication during recovery of stalled forks.

ATR-mediated activation of S-phase checkpoint
ATR is activated during every S-phase and in response to many different types of damage, including double strand breaks (DSB), base adducts, crosslinks and replication stress. The structural requirement for ATR activation is a RPA-coated single-stranded DNA with a 5 double stranded primer junction. ATR recognition of the above DNA structure depends upon a protein co-factor, ATRIP (ATR-interacting protein), that regulates ATR localization and activation. The activity of ATR-ATRIP complex is directly stimulated by TOPBP1 (DNA topoisomerase II binding protein 1), which recruitment to DNA is facilitated by the 9-1-1 (Rad9-Rad1-Hus1) checkpoint clamp.
Activated ATR signals to coordinate cell cycle transitions and repair through the phosphorylation of numerous of substrates including RAD17, p53, TopBP1 (via a feed-forward signalling loop that amplifies ATR-mediated signals), the mediator protein CEP164 and the downstream effector Chk1 (checkpoint kinase 1), which is the best characterized target of the ATR activity. Recombination proteins BRCA1 (breast cancer susceptibility gene 1), WRN (Werners syndrome helicase), and BLM (Blooms syndrome helicase) are ATR sunstrates as well. ATR also phosphorylates the Fanconi-anemia protein FANCD2 to regulate inter-strand crosslink repair as well as the nucleotide excision repair protein XPA to regulate its intracellular localization. Moreover, ATR interacts with the mismatch repair protein MSH2 (mutS homolog 2) to form a signalling module and regulate the phosphorylation of Chk1 and SMC1 (structure maintenance of chromosome 1). Upon replication stress ATR also phosphorylates the Ser-139 of H2AX/H2AFX, while is associated with the tyrosine kinase oncogene BCR-ABL after genotoxic stress.

ATR-mediated stabilization of replication forks
ATR has a crucial role in the maintenance of functional replication forks independent of its function in the activation of Rad53 (yeast homolog of checkpoint kinase 2). Among substrates of ATR on the replication forks are the proteins RPA1, RPA2, MCM2-7 (minichromosome maintenance 2-7) complex, MCM10, PCNA, replication factor C, Tim (Timeless)-Tipin complex, SMARCAL1 (HARP)-a SNF2 ATP-dependent annealing helicase, and several polymerases, such as Pol alpha and Pol epsilon. Furthermore, a key target of ATR-ATRIP complex is Claspin, and is important both for S-phase checkpoint activation (via regulation of Chk1 phosphorylation) but also for replication forks stabilization (via interactions with Pol epsilon) even in normal cycling cells. ATR is found also associated with two components of the nucleosome remodelling and deacetylating complex, the chromodomain-helicase-DNA-binding protein 4 (CHD4) and the histone-deacetylase-2 (HDAC2).
ATR also functions to stabilize fragile sites. In effect of all the above, the ATRs essential function for cell viability may be to respond to abundant sources of replication stress in normal cycling cells as well as after exposure to DNA damage agents.

ATR implication in centrosomal function via:
(a) Direct interaction with NBS1 (Nijmegen breakage syndrome 1) and BRCA1 pathway.
(b) Signalling to Chk1 and control of centrosome overduplication after DNA damage.
(c) Direct phosphorylation and delocalization from centrosome of CEP63 in the presence of chromosomal breaks.

Homology

According to HomoloGene (NCBI), homologs of the human ATR gene (NP_001175.2, 2644 aa) are the followings:
- Chimpanzee (Pan troglodytes) XP_516792.2, 2646 aa
- Dog (Canis lupus familiaris) XP_534295.2, 2644 aa
- Cattle (Bos taurus) XP_581054.3, 2644 aa
- Rat (Rattus norvegicus) XP_001062084.1, 2166 aa
- Zebrafish (Danio rerio) XP_696163.3, 2638 aa

Mutations

Somatic

Single nucleotide substitutions have been described in various types of carcinomas at total frequency 2%, mostly in heterozygous form. In particular, missense mutations have been found in the PI3/4 kinase catalytic domain and in FAT domain of breast and lung cancers respectively. Coding silent mutations have also been detected in carcinomas of stomach, breast, skin and central nervous system.

Implicated in

Entity name

ATR haploinsufficiency in mismatch repair (MMR)-deficient cancers

Note

Homozygous null mutations of ATR have not been reported in human cancers even in late-stage malignant cells and it is unlikely to exist given that mutations in both alleles of ATR gene lead to cell lethality. However, ATR gene has a potential increased susceptibility to somatic mutations in tumors with defective MMR, due to the presence of an A10 mononucleotide repeat in the exon 10 protein coding region. In particular, clinical reports have demonstrated that ATR is heterozygously mutated in certain types of tumors with mismatch repair deficiencies, including malignancies of the colon, the stomach and the endometrium. Although, it is not well understood how these mutations could contribute to the tumorigenic process, lines of evidence suggest that ATR serves as a haploinsufficient tumor suppressor in mismatch repair-deficient cells. Disruption of a single ATR allele gene in MLH1-deficient background significantly increases fragile site expression, chromosomal amplifications and rearrangements. The above chromosomal instability accompanied by hypersensitivity to genotoxic stress agents, such as hydroxyurea. Furthermore, mice with ATR+/- MLH1-/- genotype are more prone to early tumor development compared with ATR+/- or MLH1-/- counterparts. More recently has been reported that the combined ATR haploinsufficiency and MMR-deficiency that lead to chromosomal instability in colon cancer cells are also enhance the sensitivity of these cells to chemotherapeutic agent 5-fluorouracil, a standard treatment for colorectal cancers. The mechanism by which this occurs remains unclear. However, some findings suggest that MMR-deficient cells with partial reduced ATR activity are more prone to formation of DNA double strand breaks (DSBs). Additionally, partial inhibition of ATR has been reported to be significant for treatment of patients with high-microsatellite instability (MSI, a class of genomic instability clinically distinct from chromosome instability that is the result of mutations in MMR machinery) colorectal tumors increasing the disease-free survival time.

Prognosis

Recent works have demonstrated that high-MSI endometrioid endometrial cancers harboring ATR mutations have worse survival compared to ATR wild-type high-MSI tumors, suggesting that the last ones might also have an improved prognosis compared to microsatellite stable (MSS) endometrial tumors. However, the prognostic significance of ATR mutations in MMR deficient cancers remains to be clarified.

Cytogenetics

ATR partial knockdown in colon cancer cell lines with defective MMR leads to the formation of chromosomal breaks and gaps, chromosome bridges and micronuclei, as well as to the formation of supernumerary centrosomes.

Entity name

ATR-mutated Seckel syndrome (ATR-SS)

Note

Seckel syndrome is a rare autosomal recessive disorder characterised by severe intrauterine growth retardation, profound microcephaly, dwarfism, mental retardation and isolated skeletal abnormalities. At least four distinct Seckel syndrome-causative genomic loci (Skl 1-4) have been identified but only two genetic defects are known in this disorder. Both of them impacting on ATR-dependent DNA damage signalling. The first identified defect was in ATR gene itself, concerning a hypomorphic single synonymous mutation (A>G 2101) that caused aberrant splicing of ATR. Pericentrin (PCNT), a core structural component of centrosomes, has also been identified as Seckel syndrome-causative gene.

Cytogenetics

Cells from ATR-SS patients exhibit increased sensitivity to DNA replication fork stalling (measured as nuclear fragmentation and micronucleus formation), impaired phosphorylation of ATR substrates, defective G2/M arrest and supernumerous mitotic centrosomes.
Impaired ATR signalling is also characteristic of cells derived from other disorders with microcephaly and growth delay such as pericentrin-mutated Seckel syndrome (PCNT-SS), primary autosomal recessive microcephaly (MCPH) and Nijmegen breakage syndrome.

Article Bibliography

Pubmed ID	Last Year	Title	Authors
16783362	2006	Regulation of mitotic entry by microcephalin and its overlap with ATR signalling.	Alderton GK et al
15314022	2004	ATR couples FANCD2 monoubiquitination to the DNA-damage response.	Andreassen PR et al
19793861	2009	The annealing helicase SMARCAL1 maintains genome integrity at stalled replication forks.	Bansbach CE et al
11418864	2001	ATR/ATM-mediated phosphorylation of human Rad17 is required for genotoxic stress responses.	Bao S et al
12814551	2003	ATR kinase activity regulates the intranuclear translocation of ATR and RPA following ionizing radiation.	Barr SM et al
17468739	2007	DNA damage induces Chk1-dependent centrosome amplification.	Bourke E et al
10691732	2000	ATR disruption leads to chromosomal fragmentation and early embryonic lethality.	Brown EJ et al
15833913	2005	Functional uncoupling of MCM helicase and DNA polymerase activities activates the ATR-dependent checkpoint.	Byun TS et al
12526805	2002	ATR regulates fragile site stability.	Casper AM et al
12766152	2003	Human claspin is required for replication checkpoint control.	Chini CC et al
18594563	2008	ATR: an essential regulator of genome integrity.	Cimprich KA et al
9427750	1998	Overexpression of a kinase-inactive ATR protein causes sensitivity to DNA-damaging agents and defects in cell cycle checkpoints.	Cliby WA et al
15210935	2004	Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases.	Cortez D et al
11721054	2001	ATR and ATRIP: partners in checkpoint signaling.	Cortez D et al
14871897	2004	Recruitment of the cell cycle checkpoint kinase ATR to chromatin during S-phase.	Dart DA et al
15050919	2004	BCR/ABL translocates to the nucleus and disrupts an ATR-dependent intra-S phase checkpoint.	Dierov J et al
15758953	2005	Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage.	Falck J et al
15282542	2004	ATR functions as a gene dosage-dependent tumor suppressor on a mismatch repair-deficient background.	Fang Y et al
19230642	2009	ATR/Mec1: coordinating fork stability and repair.	Friedel AM et al
17344846	2007	Patterns of somatic mutation in human cancer genomes.	Greenman C et al
18157127	2008	Mutations in pericentrin cause Seckel syndrome with defective ATR-dependent DNA damage signaling.	Griffith E et al
10597277	1999	ATR is a caffeine-sensitive, DNA-activated protein kinase with a substrate specificity distinct from DNA-PK.	Hall-Jackson CA et al
11865061	2002	Hypoxia links ATR and p53 through replication arrest.	Hammond EM et al
19570909	2009	Reduced ATR or Chk1 expression leads to chromosome instability and chemosensitization of mismatch repair-deficient colorectal cancer cells.	Jardim MJ et al
19470916	2009	ATR mutations in endometrial cancer: a window into the role of mismatch repair defects.	Kauff ND et al
19473885	2009	Human DNA damage response and repair deficiency syndromes: linking genomic instability and cell cycle checkpoint proficiency.	Kerzendorfer C et al
10608806	1999	Substrate specificities and identification of putative substrates of ATM kinase family members.	Kim ST et al
17879369	2007	Mutations in the ataxia telangiectasia and rad3-related-checkpoint kinase 1 DNA damage response axis in colon cancers.	Lewis KA et al
16103057	2005	Heterozygous ATR mutations in mismatch repair-deficient cancer cells have functional significance.	Lewis KA et al
10859164	2000	Chk1 is an essential kinase that is regulated by Atr and required for the G(2)/M DNA damage checkpoint.	Liu Q et al
18851837	2008	Mrc1 and DNA polymerase epsilon function together in linking DNA replication and the S phase checkpoint.	Lou H et al
19889979	2009	Functional genomic screens identify CINP as a genome maintenance protein.	Lovejoy CA et al
11470508	2001	Evidence for alternate splicing within the mRNA transcript encoding the DNA damage response kinase ATR.	Mannino JL et al
11691784	2001	Somatic mutations in the DNA damage-response genes ATR and CHK1 in sporadic stomach tumors with microsatellite instability.	Menoyo A et al
17384679	2007	Frequent alteration of DNA damage signalling and repair pathways in human colorectal cancers with microsatellite instability.	Miquel C et al
19782648	2009	Mouse models for ATR deficiency.	O'Driscoll M et al
17531546	2007	The ATR pathway: fine-tuning the fork.	Paulsen RD et al
18371340	2007	Deletion of the developmentally essential gene ATR in adult mice leads to age-related phenotypes and stem cell loss.	Ruzankina Y et al
10545197	1999	Molecular association between ATR and two components of the nucleosome remodeling and deacetylating complex, HDAC2 and CHD4.	Schmidt DR et al
18593882	2008	Separate roles for the DNA damage checkpoint protein kinases in stabilizing DNA replication forks.	Segurado M et al
19244116	2009	Inactivation of the Nijmegen breakage syndrome gene leads to excess centrosome duplication via the ATR/BRCA1 pathway.	Shimada M et al
19158338	2009	ATR signaling at a glance.	Shiotani B et al
18283122	2008	Cep164 is a mediator protein required for the maintenance of genomic stability through modulation of MDC1, RPA, and CHK1.	Sivasubramaniam S et al
19182792	2009	An ATM- and ATR-dependent checkpoint inactivates spindle assembly by targeting CEP63.	Smith E et al
19805627	2009	Tim-Tipin dysfunction creates an indispensible reliance on the ATR-Chk1 pathway for continued DNA synthesis.	Smith KD et al
9590286	1998	Duplication of ATR inhibits MyoD, induces aneuploidy and eliminates radiation-induced G1 arrest.	Smith L et al
11114888	2000	Functional interactions between BRCA1 and the checkpoint kinase ATR during genotoxic stress.	Tibbetts RS et al
19546241	2009	Microcephalin and pericentrin regulate mitotic entry via centrosome-associated Chk1.	Tibelius A et al
14729973	2004	Quaternary structure of ATR and effects of ATRIP and replication protein A on its DNA binding and kinase activities.	Unsal-Kaçmaz K et al
14657349	2003	MSH2 and ATR form a signaling module and regulate two branches of the damage response to DNA methylation.	Wang Y et al
11673449	2001	Histone H2AX is phosphorylated in an ATR-dependent manner in response to replicational stress.	Ward IM et al
14742437	2004	UV-induced ataxia-telangiectasia-mutated and Rad3-related (ATR) activation requires replication stress.	Ward IM et al
17431119	2007	Loss of ataxia telangiectasia mutated- and Rad3-related function potentiates the effects of chemotherapeutic drugs on cancer cell survival.	Wilsker D et al
9636169	1998	Protein kinase mutants of human ATR increase sensitivity to UV and ionizing radiation and abrogate cell cycle checkpoint control.	Wright JA et al
19470935	2009	ATR mutation in endometrioid endometrial cancer is associated with poor clinical outcomes.	Zighelboim I et al
12791985	2003	Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes.	Zou L et al

Other Information

Locus ID:

NCBI: 545
MIM: 601215
HGNC: 882
Ensembl: ENSG00000175054

Variants:

dbSNP: 545
ClinVar: 545
TCGA: ENSG00000175054
COSMIC: ATR

RNA/Proteins

Gene ID	Transcript ID	Uniprot
ENSG00000175054	ENST00000350721	Q13535
ENSG00000175054	ENST00000507148	D6RFJ6
ENSG00000175054	ENST00000515149	D6RIG7
ENSG00000175054	ENST00000515810	H0Y8R8
ENSG00000175054	ENST00000656590	A0A590UJ01
ENSG00000175054	ENST00000661310	Q13535

Expression (GTEx)

Adipose - Subcutaneous

Adipose - Visceral

Adrenal Gland

Artery - Aorta

Artery - Tibial

Bladder

Brain - Amygdala

Brain - Anterior cingulate cortex

Brain - Caudate

Brain - Cerebellar Hemisphere

Brain - Cerebellum

Brain - Cortex

Brain - Frontal Cortex

Brain - Hippocampus

Brain - Hypothalamus

Brain - Nucleus accumbens

Brain - Putamen

Brain - Spinal cord

Brain - Substantia nigra

Breast - Mammary Tissue

Cells - Cultured fibroblasts

Cells - EBV - transformed lymphocytes

Cervix - Ectocervix

Cervix - Endocervix

Colon - Sigmoid

Colon - Transverse

Esophagus - Gastroesophageal Junction

Esophagus - Mucosa

Esophagus - Muscularis

Fallopian Tube

Heart - Atrial Appendage

Heart - Left Ventricle

Kidney - Cortex

Kidney - Medulla

Liver

Lung

Minor Salivary Gland

Muscle - Skeletal

Nerve - Tibial

Ovary

Pancreas

Pituitary

Prostate

Skin - Not Sun Exposed

Skin - Sun Exposed

Small Intestine - Terminal Ileum

Spleen

Stomach

Testis

Thyroid

Uterus

Vagina

Whole Blood

Pathways

Pathway	Source	External ID
Cell cycle	KEGG	ko04110
p53 signaling pathway	KEGG	ko04115
Cell cycle	KEGG	hsa04110
p53 signaling pathway	KEGG	hsa04115
HTLV-I infection	KEGG	ko05166
HTLV-I infection	KEGG	hsa05166
Fanconi anemia pathway	KEGG	ko03460
Fanconi anemia pathway	KEGG	hsa03460
DNA damage-induced cell cycle checkpoints	KEGG	hsa_M00691
DNA damage-induced cell cycle checkpoints	KEGG	M00691
Gene Expression	REACTOME	R-HSA-74160
Generic Transcription Pathway	REACTOME	R-HSA-212436
Transcriptional Regulation by TP53	REACTOME	R-HSA-3700989
Cell Cycle	REACTOME	R-HSA-1640170
Cell Cycle Checkpoints	REACTOME	R-HSA-69620
G2/M Checkpoints	REACTOME	R-HSA-69481
G2/M DNA damage checkpoint	REACTOME	R-HSA-69473
Activation of ATR in response to replication stress	REACTOME	R-HSA-176187
Meiosis	REACTOME	R-HSA-1500620
Meiotic synapsis	REACTOME	R-HSA-1221632
DNA Repair	REACTOME	R-HSA-73894
Cellular responses to stress	REACTOME	R-HSA-2262752
Cellular response to heat stress	REACTOME	R-HSA-3371556
Regulation of HSF1-mediated heat shock response	REACTOME	R-HSA-3371453
DNA Double-Strand Break Repair	REACTOME	R-HSA-5693532
Homology Directed Repair	REACTOME	R-HSA-5693538
HDR through Homologous Recombination (HR) or Single Strand Annealing (SSA)	REACTOME	R-HSA-5693567
Processing of DNA double-strand break ends	REACTOME	R-HSA-5693607
HDR through Homologous Recombination (HRR)	REACTOME	R-HSA-5685942
Homologous DNA Pairing and Strand Exchange	REACTOME	R-HSA-5693579
Presynaptic phase of homologous DNA pairing and strand exchange	REACTOME	R-HSA-5693616
HDR through Single Strand Annealing (SSA)	REACTOME	R-HSA-5685938
Fanconi Anemia Pathway	REACTOME	R-HSA-6783310
TP53 Regulates Transcription of DNA Repair Genes	REACTOME	R-HSA-6796648
Regulation of TP53 Activity	REACTOME	R-HSA-5633007
Regulation of TP53 Activity through Phosphorylation	REACTOME	R-HSA-6804756

PharmGKB

Entity ID	Name	Type	Evidence	Association	PK	PD	PMIDs
PA110	CHEK1	Gene	Pathway	associated
PA404	CHEK2	Gene	Pathway	associated

References

Pubmed ID	Year	Title	Citations
37806848	2024	TuBG1 promotes hepatocellular carcinoma via ATR/P53-apoptosis and cycling pathways.	2
37930853	2024	ATR phosphorylates DHX9 at serine 321 to suppress R-loop accumulation upon genotoxic stress.	4
38554113	2024	SIRT2 promotes base excision repair by transcriptionally activating OGG1 in an ATM/ATR-dependent manner.	0
38721777	2024	EXO1 and DNA2-mediated ssDNA gap expansion is essential for ATR activation and to maintain viability in BRCA1-deficient cells.	0
37806848	2024	TuBG1 promotes hepatocellular carcinoma via ATR/P53-apoptosis and cycling pathways.	2
37930853	2024	ATR phosphorylates DHX9 at serine 321 to suppress R-loop accumulation upon genotoxic stress.	4
38554113	2024	SIRT2 promotes base excision repair by transcriptionally activating OGG1 in an ATM/ATR-dependent manner.	0
38721777	2024	EXO1 and DNA2-mediated ssDNA gap expansion is essential for ATR activation and to maintain viability in BRCA1-deficient cells.	0
36749285	2023	The "extreme phenotype approach" applied to male breast cancer allows the identification of rare variants of ATR as potential breast cancer susceptibility alleles.	0
37144518	2023	Replisome dysfunction upon inducible TIMELESS degradation synergizes with ATR inhibition to trigger replication catastrophe.	3
37227155	2023	The FBXO32/ATR/ATM axis acts as a molecular switch to control the sensitivity of osteosarcoma cells to irradiation through its regulation of EXO1 expression.	1
37338595	2023	Expression variations of DNA damage response genes ATM and ATR in blood cancer patients.	0
37378431	2023	Alteration of replication protein A binding mode on single-stranded DNA by NSMF potentiates RPA phosphorylation by ATR kinase.	2
37454295	2023	ATR protects ongoing and newly assembled DNA replication forks through distinct mechanisms.	5
37592023	2023	Exome sequencing identifies breast cancer susceptibility genes and defines the contribution of coding variants to breast cancer risk.	7

Citation

Mary E Gagou ; Mark Meuth

ATR (ataxia telangiectasia and Rad3 related)

Atlas Genet Cytogenet Oncol Haematol. 2010-05-01

Online version: http://atlasgeneticsoncology.org/gene/728