2016-12-01   Monireh Arjmand 

Department of Biology, Faculty of Science, Payame Noor University, Tehran, Iran. bhr.arjmand@gmail.com


Atlas Image


MicroRNAs (miRNAs) are a class of small regulatory RNAs (20-23 nucleotides long) that have been found to play a critical role in the wide spectrum of biological processes. These small endogenous noncoding RNAs are able to negatively regulate gene expression by pairing with the 3-untranslated region (3-UTR) of their target mRNA. miR-150 is a short and single-stranded miRNA, encoded by a gene located at 19q13.33 in Homo sapiens, which is processed to two mature forms (miR-150-5p and miR-150-3p). It has proven to be a pivotal miRNA engaged in the development of hematologic malignancies, normal haematopoiesis, differentiation of B and T cells, and a number of tumours including breast, osteosarcoma, lung, gastric cancer and etc.


Atlas Image


RNA polymerase II produces the primary miRNA transcripts (Lee et al., ?2004) what will result in hairpin loop structures by microprocessor complex (Cai et al., ?2004; Lee et al., ?2003; Gregory et al., ?2006). The stem-loop structure has a two-nucleotide overhang at its 3 end and termed as a pre-miRNA (precursor-miRNA). Pre-miRNAs will be exported to cytoplasm through the nucleocytoplasmic shuttler Exportin-5 (XPO5), in an energy dependent mechanism (Auyeung et al., 2013; Murchison et al., ?2004). In the cytoplasm, the pre-miRNA hairpin is cleaved to a miRNA:miRNA* duplex by the endoribonuclease Dicer (Lund et al., ?2006; Park et al., ?2011). Potentially, either strand of the duplex could be a functional miRNA, however only one strand usually interacts with mRNA target through the RNA-induced silencing complex (RISC) (Filipowicz et al., ?2008). The sequence of has-miRNA-150, through the above mentioned mechanism, will be transcribed to a stem-loop structure (Reuter et al., ?2010) which finally will maturates to MIR150-5p and MIR150-3p.



microRNAs are not translated into amino acids.

Implicated in

Top note
There are growing reports on regulatory roles of small RNAs including transcriptional gene silencing/activation and post-transcriptional gene silencing events (Thomas et al., 2007; Nassiri et al. 2013a; Nassiri et al. 2013b; Azimzadeh et al., ?2014). The role of MIR150 as an oncogene or tumor suppressor in different tumors and its potential as a tumor biomarker of prognosis, ad for targeted therapeutic strategy, is been investigated (Wang et al., ?2015).
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MIR150 drives megakaryocyte-erythrocyte progenitors (MEPs) differentiation toward megakaryocytes at the expense of erythroid cells, and the transcription factor MYB is a critical target of MIR150 in this regulation (Lu et al., ?2008). Thrombopoietin ( THPO) down-modulates MYB expression through induction of MIR150 (Barroga et al., ?2008). MIR150 is expressed in mature B and T cells and regulates their differentiation; MIR150 blocks the transition of pro-B to pre-B cells (Xiao et al., ?2007).
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Cell-cell signaling
Some cells secrete miRNAs as cell-cell signaling molecules (intercellular/interorgan) (Azimzadeh et al., ?2014). Human THP-1 cells selectively package MIR150 into active microvesicles in vitro. These secreted microvesicles are delivered specifically into human HMEC-1 cells and induce their migration as an inflammatory response (Zhang et al. 2010).
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Pancreatic Cancer
MUC4, a member of Mucins family, is a glycoprotein upregulated in pancreatic tumors. Silencing of MUC4 results in the suppression of cell growth and metastasis in pancreatic tumors (Singh et al., ?2004). The binding site of MIR150 is located at the 3 UTR of MUC4, which leads to the downregulation of MUC4 expression. The overexpression of MIR150 mitigates the malignant behavior of pancreatic cancer cells (Srivastava et al., 2011).
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Colorectal cancer
In a work aiming to figure out the altered miRNAs in colorectal cancer (CRC) through characterization of the miRNA profiles of serum exosomes, Ogata-Kawata et al. has showed that the serum exosomal levels of seven miRNAs, including MIR150, were significantly higher in primary CRC patients compared to healthy controls, and were significantly down-regulated after tumor surgery (Ogata-Kawata et al., ?2014). However, miRNA expression profiling using qRT-PCR assays in 1) normal colorectal tissues, 2) adenoma and 3) CRC, MIR150 was found low down-regulated in all three groups and tumour tissue had reduced levels of MIR150 expression compared with paired non-cancerous tissue (Ma et al., ?2012).
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Esophageal cancer
MIR150 expression is significantly lower in esophageal squamous cell carcinoma (ESCC) samples compared with the normal esophageal mucosa levels (P < 0.001) (Yokobori et al., ?2013). It has been supposed that MIR150 suppresses tumor proliferation by degradation the Zinc finger E box binding homeobox 1 ( ZEB1).
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Gastric cancer
The probability of survival in gastric cancer is significantly lower in patients with high expression levels of MIR150 (Katada et al., 2009). MIR150 increases the proliferation rate of gastric cancer cells by targeting the tumor-suppressor early growth response factor 2 (EGR2) (Wu et al., ?2010).
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Breast cancer
High levels of MIR150 have been found in breast cancer tissues. The application of MIR150 inhibitors against breast cancer cell lines leads to apoptosis. There is a highly conserved MIR150-binding motif at the 3UTR of P2X7 receptor (P2RX7 ID: 41623> ); MIR150 down-regulates the P2X7 protein levels and therefore causes the cancerous cells to escape from P2X7 pro-apoptotic control (Huang et al., ?2013).
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Lung cancer
Application of antisense oligonucleotide (ASO) against MIR150 in lung carcinoma cells A549 has induced the reduction of cell proliferation rates and increased apoptosis. Up-regulation of TP53 was detected after ASO treatment (Li et al., ?2012). MIR150 is upregulated in lung cancer and correlates negatively with the expression of TP53 (Zhang et al., ?2013). MIR150 regulates the expression of TP53 through binding to its 3-UTR, thus inhibition of MIR150 leads to reduction of cell proliferation and increases apoptosis and expression of TP53. In another study, it was demonstrated that MIR150 represses SRC Kinase Signaling Inhibitor 1 (SRCIN1) which leads to the activation of the Src/focal adhesion kinase (PTK2 or FAK) and Src/Ras/extracellular signal-regulated kinase (ERK) pathway, and enhances the migration and proliferation rate of A549 cells (Cao et al., ?2014). Moreover, GU XY et al. showed that the overexpression of MIR150 in non-small-cell lung cancer (NSCLC) is associated with alterations in the expression of human BRI1-associated receptor kinase 1 (BAK1), so that inhibition of MIR150 reduces cell proliferation and promotes cell apoptosis (Gu et al., ?2014). However, it has been argued that the expression level of MIR150 in patients with small-cell lung cancer (SCLC) is much lower than normal lung samples (Bi et al., ?2014).
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Liver cancer
It has been suggested that MIR150 could be a suitable candidate to discriminate tumoural versus normal human primary hepatocytes (Di Masi et al., ?2010). In addition, it has been confirmed that the overexpression of MIR150 downregulates MYB protein levels and causes a significant reduction of CD133+ cells and the suppression of cell growth/tumorsphere formation (Zhang et al., ?2012). It is suggested that MIR150 may be engaged in the self-renewal of liver cancer stem cells through modulation of the downstream target MYB.
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A nearly 50-fold overexpression of MIR150 in osteosarcoma samples compared to normal osteoblasts, has been reported (Lulla et al., ?2011). Upregulation of MIR150 may cause the down-regulation of P2X7 receptor and therefore an uncontrolled growth of osteosarcoma cells. Other study has argued that MIR150 inhibits cell proliferation and metastasis in osteosarcoma and stimulates cell apoptosis by regulating the expression of SP1 (Li et al., ?2015). Recently, it has been reported that MIR150 functions as a tumor suppressor in osteosarcoma partially by targeting the IGF2 mRNA-binding protein 1 (IGF2BP1) (Qu et al., ?2016).
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Hepatocellular carcinoma
In a recent attempt to identifying the role of miRNAs in hepatocellular carcinoma (HCC) progression, researchers have shown that there are low levels of miR-150-5p in cancerous samples, and determined that the inhibition of miR-150-5p has a drastic effect on hepatoma cell migration and invasion, whereas its overexpression shows reverse results (Li et al., ?2014). It was also confirmed that miR-150-5p inhibits the matrix metalloproteinase 14 (MMP14) expression in hepatoma cells.
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Prostate cancer
The high expression levels of MIR150 is positively correlated with tumor recurrence or metastasis (p=0.010) (Dezhong et al., ?2015). It was shown that prostate cancer patients with high MIR150 expression have significantly poorer overall survival and poorer disease-free survival compared those with low MIR150 expression. Furthermore, increased MIR150 expression might participate in the development and progression of human prostate cancer stem cells (CSC) via suppressing p27Kip1 (CDKN1B ) (Liu et al., ?2015).
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Intrahepatic cholangiocarcinoma
MIR150 is significantly downregulated in intrahepatic cholangiocarcinoma (ICC) tissues; however its expression level in the blood samples of ICC patients is significantly higher than in controls (Wang et al., ?2015).
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MIR150 is dramatically down-regulated in BL patients; MYB and Survivin (BIRC5) are the targetrs of MIR150 (Wang et al., ?2014). In addition, MIR150 can induce Epstein Barr virus-positive BL differentiation by targeting MYB (Chen et al., ?2013).
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Cervical cancer
Interacting with 3 UTR of FOXO4, MIR150 reduces FOXO4 expression level and thereby regulates several cell cycle- and apoptosis- related genes (CyclinD1 (CCND1), p27, BCL2L11 (BIM), and FASLG), and leads to cervical cancer cell growth and survival (J Li et al., ?2015).
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Bladder cancer
An in vitro investigation to figuring out the role of MIR150 in cells has shown that MIR150 functions as a tumor promoter in reducing chemo-sensitivity and promoting invasiveness of muscle-invasive bladder cancer cells via targeting programmed cell death 4 protein (PDCD4) (Y Lei et al., ?2014).
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Epithelial ovarian cancer
A significant low expression level of MIR150 have been reported in epithelial ovarian cancer (EOC) tissues and patients serum (Vang et al, ?2013; Shapira et al., ?2014). MIR150 may function as a tumor suppressor and modulate EOC cell proliferation, and invasion by directly and negatively regulating Zinc Finger E-Box Binding Homeobox 1 (ZEB1) (Jin et al., ?2014).
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Patients with severe A/H1N1 disease exhibited a significant over-expression of circulating MIR150 than patients with milder disease, suggesting that the up-regulation of MIR150 is engaged with poorer outcomes of A/H1N1 infection (Morán et al., ?2015).
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Acquired immune deficiency syndrome (AIDS)
Down regulation of MIR150 during HIV infection and its lower level in CD4+ T cells of chronic HIV patients compared to healthy controls has been reported (Witwer et al., ?2012; Swaminathan et al., ?2009). It has been speculated that the suppression of MIR150 promotes HIV-1 infection (X Wang et al., ?2009). HIV positive patients can be grouped based on the MIR150 levels of their peripheral blood mononuclear cells or their plasma, suggesting MIR150 as a new biomarker for HIV progression, therapy and resistance (Munshi et al., ?2014).
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Dengue haemorrhagic fever (DHF)
The downregulation of suppressors of cytokine signaling 1 (SOCS1) proteins through transfection of a MIR150 mimic into CD14(+) cells infected with DENV-2, suggests that augmented MIR150 expression with depressed SOCS1 expression in CD14(+) cells is associated with the pathogenesis of DHF (RF Chen et al., ?2014).
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Cardiovascular Diseases
Low expression levels of MIR150 is associated with acute myocardial infarction, atrial fibrillation, dilated cardiomyopathy, ischaemic cardiomyopathy and various mouse heart failure models (Devaux et al., ?2013; Liu et al., ?2012; Topkara et al., ?2011; Duan et al., ?2013). MIR150 could be a better biomarker for HF than other markers used in clinic (Ellis et al., ?2013). MIR150 has a pivotal role as a regulator of cardiomyocyte survival during cardiac injury (Tang et al., ?2015). Reduced circulating MIR150 levels are associated with poor survival in pulmonary arterial hypertension (Rhodes et al., ?2013).
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Systemic sclerosis
MIR150 may play an important role in the pathogenesis of Systemic Sclerosis (SSc) via overexpression of integrin β 3 (ITGB3) (Honda et al., ?2013). MIR150 expression level is decreased in SSc fibroblasts and the treatment of normal fibroblasts with MIR150 inhibitor promotes the expression of integrin β3, phosphorylated SMAD3, and type I collagen. SSc patients with lower serum MIR150 levels show more severe clinical manifestations.
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Multiple sclerosis (MS)
MIR150 is down-regulated in peripheral blood mononuclear cells and T cells from MS patients (Martinelli-Boneschi et al., ?2012; Freiesleben et al., ?2016).


Pubmed IDLast YearTitleAuthors
234152312013Beyond secondary structure: primary-sequence determinants license pri-miRNA hairpins for processing.Auyeung VC et al
241977382014Human RNAi pathway: crosstalk with organelles and cells.Azimzadeh Jamalkandi S et al
188149502008Thrombopoietin regulates c-Myb expression by modulating micro RNA 150 expression.Barroga CF et al
246379272014A microRNA signature predicts survival in early stage small-cell lung cancer treated with surgery and adjuvant chemotherapy.Bi N et al
155257082004Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs.Cai X et al
244567952014miR-150 promotes the proliferation and migration of lung cancer cells by targeting SRC kinase signalling inhibitor 1.Cao M et al
249074212014Augmented miR-150 expression associated with depressed SOCS1 expression involved in dengue haemorrhagic fever.Chen RF et al
235212172013Re-expression of microRNA-150 induces EBV-positive Burkitt lymphoma differentiation by modulating c-Myb in vitro.Chen S et al
235471712013MicroRNA-150: a novel marker of left ventricular remodeling after acute myocardial infarction.Devaux Y et al
257783132015miR-150 is a factor of survival in prostate cancer patients.Dezhong L et al
209372172010Characterization of HuH6, Hep3B, HepG2 and HLE liver cancer cell lines by WNT/β - catenin pathway, microRNA expression and protein expression profile.Di Masi A et al
232117182013miR-150 regulates high glucose-induced cardiomyocyte hypertrophy by targeting the transcriptional co-activator p300.Duan Y et al
236966132013Circulating microRNAs as candidate markers to distinguish heart failure in breathless patients.Ellis KL et al
181971662008Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight?Filipowicz W et al
169573652006MicroRNA biogenesis: isolation and characterization of the microprocessor complex.Gregory RI et al
245324682014Down-regulation of miR-150 induces cell proliferation inhibition and apoptosis in non-small-cell lung cancer by targeting BAK1 in vitro.Gu XY et al
231599432013miR-150 down-regulation contributes to the constitutive type I collagen overexpression in scleroderma dermal fibroblasts via the induction of integrin β3.Honda N et al
243124952013miR-150 promotes human breast cancer growth and malignant behavior by targeting the pro-apoptotic purinergic P2X7 receptor.Huang S et al
238955172013MicroRNA regulate immune pathways in T-cells in multiple sclerosis (MS).Jernås M et al
250900052014MicroRNA-150 predicts a favorable prognosis in patients with epithelial ovarian cancer, and inhibits cell invasion and metastasis by suppressing transcriptional repressor ZEB1.Jin M et al
191484902009microRNA expression profile in undifferentiated gastric cancer.Katada T et al
145084932003The nuclear RNase III Drosha initiates microRNA processing.Lee Y et al
153720722004MicroRNA genes are transcribed by RNA polymerase II.Lee Y et al
252877162014miR-150 modulates cisplatin chemosensitivity and invasiveness of muscle-invasive bladder cancer cells via targeting PDCD4 in vitro.Lei Y et al
267153622015microRNA-150 promotes cervical cancer cell growth and survival by targeting FOXO4.Li J et al
255493552014miR-150-5p inhibits hepatoma cell migration and invasion by targeting MMP14.Li T et al
263612182015MicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcoma.Li X et al
219355782012Regression of A549 lung cancer tumors by anti-miR-150 vector.Li YJ et al
266365222015MIR-150 promotes prostate cancer stem cell development via suppressing p27Kip1.Liu DZ et al
230286712012The expression levels of plasma micoRNAs in atrial fibrillation patients.Liu Z et al
185391142008MicroRNA-mediated control of cell fate in megakaryocyte-erythrocyte progenitors.Lu J et al
217890312011Identification of Differentially Expressed MicroRNAs in Osteosarcoma.Lulla RR et al
173812812006Substrate selectivity of exportin 5 and Dicer in the biogenesis of microRNAs.Lund E et al
220520602012miR-150 as a potential biomarker associated with prognosis and therapeutic outcome in colorectal cancer.Ma Y et al
221085672012MicroRNA and mRNA expression profile screening in multiple sclerosis patients to unravel novel pathogenic steps and identify potential biomarkers.Martinelli-Boneschi F et al
261489292015Circulating levels of miR-150 are associated with poorer outcomes of A/H1N1 infection.Morán J et al
248283362014MicroRNA-150 is a potential biomarker of HIV/AIDS disease progression and therapy.Munshi SU et al
151453452004miRNAs on the move: miRNA biogenesis and the RNAi machinery.Murchison EP et al
247052492014Circulating exosomal microRNAs as biomarkers of colon cancer.Ogata-Kawata H et al
217538502011Dicer recognizes the 5' end of RNA for efficient and accurate processing.Park JE et al
265614652016MicroRNA-150 functions as a tumor suppressor in osteosarcoma by targeting IGF2BP1.Qu Y et al
202306242010RNAstructure: software for RNA secondary structure prediction and analysis.Reuter JS et al
232209122013Reduced microRNA-150 is associated with poor survival in pulmonary arterial hypertension.Rhodes CJ et al
243662982014Circulating biomarkers for detection of ovarian cancer and predicting cancer outcomes.Shapira I et al
147447772004Inhibition of MUC4 expression suppresses pancreatic tumor cell growth and metastasis.Singh AP et al
219831272011MicroRNA-150 directly targets MUC4 and suppresses growth and malignant behavior of pancreatic cancer cells.Srivastava SK et al
194436732009Does the presence of anti-HIV miRNAs in monocytes explain their resistance to HIV-1 infection?Swaminathan S et al
258241472015MicroRNA-150 protects the mouse heart from ischaemic injury by regulating cell death.Tang Y et al
172938652007High-throughput oncogene mutation profiling in human cancer.Thomas RK et al
214313052011Role of microRNAs in cardiac remodeling and heart failure.Topkara VK et al
235548782013Identification of ovarian cancer metastatic miRNAs.Vang S et al
261709692015Role of microRNA-150 in solid tumors.Wang F et al
246136882014Low expression of miR-150 in pediatric intestinal Burkitt lymphoma.Wang M et al
254823202015Upregulated circulating miR-150 is associated with the risk of intrahepatic cholangiocarcinoma.Wang S et al
190153952009Cellular microRNA expression correlates with susceptibility of monocytes/macrophages to HIV-1 infection.Wang X et al
222402562012Relationships of PBMC microRNA expression, plasma viral load, and CD4+ T-cell count in HIV-1-infected elite suppressors and viremic patients.Witwer KW et al
200677632010MiR-150 promotes gastric cancer proliferation by negatively regulating the pro-apoptotic gene EGR2.Wu Q et al
179230942007MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb.Xiao C et al
230131352013MiR-150 is associated with poor prognosis in esophageal squamous cell carcinoma via targeting the EMT inducer ZEB1.Yokobori T et al
220252692012microRNA-150 inhibits human CD133-positive liver cancer stem cells through negative regulation of the transcription factor c-Myb.Zhang J et al
237473082013miR-150 promotes the proliferation of lung cancer cells by targeting P53.Zhang N et al
206030812010Secreted monocytic miR-150 enhances targeted endothelial cell migration.Zhang Y et al

Other Information

Locus ID:

NCBI: 406942
MIM: 611114
HGNC: 31537
Ensembl: ENSG00000207782


dbSNP: 406942
ClinVar: 406942
TCGA: ENSG00000207782



PathwaySourceExternal ID
MicroRNAs in cancerKEGGhsa05206
MicroRNAs in cancerKEGGko05206


Pubmed IDYearTitleCitations
200677632010MiR-150 promotes gastric cancer proliferation by negatively regulating the pro-apoptotic gene EGR2.89
230796612012Blockade of miR-150 maturation by MLL-fusion/MYC/LIN-28 is required for MLL-associated leukemia.82
215512312011Modulation of microRNA expression in human T-cell development: targeting of NOTCH3 by miR-150.77
219831272011MicroRNA-150 directly targets MUC4 and suppresses growth and malignant behavior of pancreatic cancer cells.64
279039782017Circular RNA ZNF609 functions as a competitive endogenous RNA to regulate AKT3 expression by sponging miR-150-5p in Hirschsprung's disease.54
244567952014miR-150 promotes the proliferation and migration of lung cancer cells by targeting SRC kinase signalling inhibitor 1.49
220252692012microRNA-150 inhibits human CD133-positive liver cancer stem cells through negative regulation of the transcription factor c-Myb.48
186823932008MicroRNAs miR-186 and miR-150 down-regulate expression of the pro-apoptotic purinergic P2X7 receptor by activation of instability sites at the 3'-untranslated region of the gene that decrease steady-state levels of the transcript.47
237473082013miR-150 promotes the proliferation of lung cancer cells by targeting P53.47
230131352013MiR-150 is associated with poor prognosis in esophageal squamous cell carcinoma via targeting the EMT inducer ZEB1.46


Monireh Arjmand


Atlas Genet Cytogenet Oncol Haematol. 2016-12-01

Online version: http://atlasgeneticsoncology.org/gene/50556/mir150