PRKAA1 (protein kinase AMP-activated catalytic subunit alpha 1)

2018-07-01   Esin Gülce Seza  , Ismail Güderer  , Çagdas Ermis  , Sreeparna Banerjee  

Department of Biology, Middle East Technical University, 06800 Ankara, Turkey; banerjee@metu.edu.tr

Identity

HGNC
PRKAA1
LOCATION
5p13.1
LOCUSID
5562
ALIAS
AMPK,AMPK

Abstract

Protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), also known as AMPK α1, is an energy sensor that plays a key role in the regulation of cellular energy metabolism. AMPK α1 is the catalytic subunit of the heterotrimeric AMPK protein with a length of 548 amino acids. A key switch to activate this protein is an alteration in the AMP\/ATP ratio. The protein is dysregulated in several human diseases including diabetes and metabolic syndrome, cardiovascular diseases, neurodegenerative diseases and many cancer types (Steinberg and Kemp, 2009). Two isoforms of AMPK exist including AMPK α1 and AMPK α2; however, discrimination between these isoforms for their involvement in certain diseases is currently not possible.

DNA/RNA

Note

Detailed genomic configuration of human PRKAA1 gene can be found in https://www.ncbi.nlm.nih.gov/gene/5562.

Description

The human AMPK α1 gene is located on 5p13.1 and spans about 39 kb. It contains 12 exons and 2 promoters named as PRKAA1_1 and PRKAA1_2. The gene has 3 isoforms named as PRKAA1_001, PRKAA1_002 and PRKAA1_003.

Transcription

The human AMPK α1 gene has 9 transcripts: PRKAA1-201 (1134 bp), PRKAA1-202 (1918 bp), PRKAA1-204 (5088 bp) that code for a protein. PRKAA1-203 (425 bp), PRKAA1-205 (919 bp), PRKAA1-206 (1082 bp), PRKAA1-207 (692 bp), PRKAA1-208 (668 bp) and PRKAA1-209 (436 bp) have retained introns. It also has 7 paralogues and 97 orthologues.

Pseudogene

PRKAA1 has one hypothetical pseudogene titled as LOC363815 from Rattus norvegius and is located in 11q23.

Proteins

Description

AMPK α1 is the catalytic subunit of the heterotrimeric AMPK protein with a length of 548 amino acids. In response to an increase in the AMP/ATP ratio, AMPK gets activated. AMP binds to the non-catalytic gamma subunit of the AMPK protein and induces phosphorylation of Thr-183 (Lizcano et al., 2004). This residue is present in the T-loop region of the catalytic subunit, AMPK α1 (Bright et al., 2009).
There are several known AMPK kinases (AMPKKs). STK11 (LKB1), complexed with STRADA and CAB39 (MO25), is the major upstream regulator of the AMPK, which phosphorylates the AMP bound protein (Shackelford and Shaw, 2009). Ca2+/calmodulin-dependent protein kinase kinase β ( CAMKK2 or CaMKKβ) is also known to be an upstream kinase of AMPK (Sundararaman et al., 2016). TGF-beta-activated kinase-1 ( MAP3K7 or TAK1) may also phosphorylate AMPK α or at least play a role in its activation as loss of TAK1 leads to impaired AMPK activation (Xie et al., 2006).
The AMPK α1 protein consists of several domains (Figure 1). The N-terminal kinase domain carries out the serine/threonine kinase function. The C-terminus regulatory domain contains an α-RIM sensor loop and a β-subunit interaction domain (Crute et al., 1998). A UBA-like auto-inhibitory domain (AID) is present between the α-RIM sensor loop and the kinase domain. AID is required for allosteric regulation via AMP. Absence of this inhibitory region renders the protein independent of AMP but still requires phosphorylation of the activation loop (Crute et al., 1998).
Atlas Image
Figure 1. Domains of AMPK-α1. (AID: UBA-like Autoinhibitory Domain)

Expression

AMPK α1 is widely expressed across many tissues such as brain, heart, kidney, liver and lung (Stapleton et al., 1996).

Localisation

It is primarily localized in the cytoplasm, and with HUVEC cells it was shown that AMPK α1 localizes exclusively in the cytoskeleton (Pinter et al., 2012).

Function

AMPK α1, in its active form, phosphorylates many downstream proteins. These phosphorylated target proteins of AMPK regulate metabolism, autophagy, cell growth and proliferation, and cell polarity (Hardie, 2011). AMPK exists as an obligate heterotrimer in cells (Mihaylova and Shaw, 2011), and all the functions that will be mentioned in this section are carried out by the α1 subunit in this obligate heterotrimer complex.
  • Cellular Metabolism
    AMPK is activated when there is energy stress in the cell manifested by an increase in the AMP/ATP ratio. In response to this stress, AMPK activates catabolic pathways while inhibiting anabolic pathways.
    • Glycolysis
      One of the key catabolic pathways for energy generation, glycolysis, is upregulated through AMPK signalling. In order increase glucose uptake to the cell, AMPK activates (induces translocation, short term response) and increases protein expression (longer term response) of SLC2A1 (GLUT1) and SLC2A4 (GLUT4) (Fryer et al., 2002). Also, 6-phosphofructo-2-kinase ( PFKFB3 or PFK-2) gets phosphorylated and activated by AMPK which enhances glycolysis (Marsin et al., 2000). Glycogen synthesis (anabolic pathway) is inhibited by the phosphorylation of glycogen synthase.
    • Gluconeogenesis
      Anabolic pathways such as gluconeogenesis that enhance glucose levels are inhibited by repression of transcripts that encode for gluconeogenesis enzymes. CRTC2, coactivator of the cyclic AMP response element-binding protein CREB, gets phosphorylated and inhibited (excluded from the nucleus) by AMPK. This leads to disruption of CREB-CRTC2 complex and inhibition of CREB-dependent gluconeogenesis (Lee et al., 2010). Transcription of mRNAs encoding glucose-6-phosphatase and phosphoenolpyruvate carboxykinase are inhibited via this mechanism. Also, class IIA histones, which can activate the FOXO family of transcription factors via HDAC3 recruitment, gets phosphorylated and excluded from the nucleus. This decrease in activity of FOXO family of transcription factors leads to reduced expression of gluconeogenesis genes (Mihaylova et al., 2011).
    • Lipid Metabolism
      In AMPK activated cells, fatty acid uptake is increased by translocation of fatty acid translocase, CD36 (FAT), to the cellular membrane (Bonen et al., 2007). Meanwhile, acetyl-CoA carboxylase ( ACACA ACC1), which catalyses the rate-limiting step of fatty acid synthesis (Hofbauer et al., 2014), gets phosphorylated and this phosphorylation inhibits the enzymatic activity of ACC1. Along with CD36 (FAT) translocation to the membrane, ACACB (ACC2) is also inhibited which leads to increased fatty acid uptake into mitochondria due to decreased amounts of malonyl-CoA in the cell (Merrill et al., 1997).
    • Protein Synthesis
      Synthesis of proteins is an enormous energy consuming process for the cells. MTOR, in its active form, promotes cell proliferation and protein synthesis. Activated AMPK inhibits mTOR via phosphorylation of upstream regulator TSC2 (Huang and Manning, 2008) and its subunit RPTOR (Raptor) (Gwinn et al., 2008). Also, eukaryotic elongation factor 2 ( EEF2) is required for the elongation of translation in eukaryotes. EEF2 kinase gets activated by AMPK which inhibits EEF2 via phosphorylation, resulting in inhibition of protein synthesis (Horman et al., 2002).
  • Autophagy
    Excess or dysfunctional organelles get "eaten up" by the cell over time, this process is called autophagy and it can give cells the advantage of recycling important nutrients, especially during starvation. It is known that mTORc1 inhibits autophagy via inhibition of ULK1 (Chan, 2009), and AMPK downregulates mTORc1 via phosphorylation of TSC2 and Raptor. This was thought to be the main mechanism by which AMPK activates autophagy. Recently, it was found that initiator of autophagy, the ULK1 protein kinase, directly interacts with AMPK, and gets phosphorylated and activated by AMPK (Roach, 2011).
  • Cell Growth and Proliferation
    AMPK can act as a metabolic checkpoint via inhibition of cellular growth when energy status in the cell is compromised (Mihaylova and Shaw, 2011). Processes of cellular growth and proliferation require many events to take place in the cell such as protein and lipid synthesis. As mentioned above, AMPK can decrease the synthesis of proteins and subsequently cell proliferation through the inhibition of mTORc1.
    mTORc1 also controls lipid biosynthesis via a transcription factor named as sterol regulatory element-binding protein-1, SREBF1 (SREBP-1) (Laplante and Sabatini, 2009). SREBP-1 targets lipogenic genes such as ACC (Brown et al., 2007); fatty acid synthase, FASN (Jung et al., 2012); and stearoyl-CoA desaturase 1, SCD (Mauvoisin et al., 2007). mTORc1 inhibition by AMPK along with the previously mentioned inhibition of ACC1 leads to decreased lipid synthesis in the cell.
    Other than metabolic effects, AMPK also activates checkpoint regulators such as TP53 via inactivation of SIRT1 (Sirtuin 1) (Lee et al., 2012) and phosphorylation at Ser-15 (Jones et al., 2005), as well as CDKN1B (cyclin-dependent kinase inhibitor p27(Kip1)) via phosphorylation at Thr198 (Liang et al., 2007).
  • Cell Polarity
    LKB1-null and AMPK-null Drosophila models show lethal phenotypes with severe defects in cell polarity and mitosis (Lee at al., 2007). AMPK activation was reported to rescue LKB1-null phenotype while non-muscle myosin regulatory light chain (MRLC) phopshomimetic mutants rescued AMPK-null models (Lee at al., 2007). However, another study reported that in mammalian MDCK cells, AMPK activation did not change phosphorylation of MRLC, rather AFDN (afadin) was identified as AMPK substrate for phosphorylation (Zhang et al., 2011). Activation via AMPK leads to deposition of junction components in the cellular membrane.
    The microtubule plus-end-tracking protein CLIP1 (CLIP-170) is activated via phosphorylation by AMPK. CLIP-170 phosphorylation is required for microtubule dynamics and the regulation of directional cell migration (Nakano et al., 2010). The same study reported that inhibition of AMPK leads to accumulation of CLIP-170 at microtubule tips and slower tubulin polymerization (Nakano et al., 2010). Thus, AMPK also controls microtubule dynamics through CLIP-170 phosphorylation.
Atlas Image
Figure 2. Functions of AMPK

Homology

AMPK α1, with its kinase and regulatory domains, is a very well conserved protein.
Homologs of Human PRKAA1 (AMPK α1)
  Gene Name

  Organism

  NCBI RefSeq

  Protein

  Length (aa)

  PRKAA1

  H. sapiens

  NP_996790.3

  5-AMP-activated protein kinase catalytic subunit alpha-1

  574

  PRKAA1

   P. troglodytes

  XP_009447514.1

  5-AMP-activated protein kinase catalytic subunit alpha-1

  574

  PRKAA1

  M. mulatta

  XP_001086410.2

  5-AMP-activated protein kinase catalytic subunit alpha-1

  559

  PRKAA1

  C. lupus

  XP_022273603.1

  5-AMP-activated protein kinase catalytic subunit alpha-1

  573

  PRKAA1

  B. taurus

  NP_001103272

  5-AMP-activated protein kinase catalytic subunit alpha-1

  458

  Prkaa1

  M. musculus

  NP_001013385.3

  5-AMP-activated protein kinase catalytic subunit alpha-1

  559

  Prkaa1

  R. norvegicus

  NP_062015.2

  5-AMP-activated protein kinase catalytic subunit alpha-1

  559

  PRKAA1

  G. gallus

  NP_001034692.1

  5-AMP-activated protein kinase catalytic subunit alpha-1

  560

  prkaa1

  X. tropicalis

  NP_001120434.1

  5-AMP-activated protein kinase catalytic subunit alpha-1

  551

  prkaa1

  D. rerio

  NP_001103756.1

  5-AMP-activated protein kinase catalytic subunit alpha-1

  573

  KIN10

  A. thaliana

  NP_001118546.1

  SNF1 kinase homolog 10

  512

  KIN11

  A. thaliana

  NP_974374.1

  SNF1 kinase homolog 11

  512

  Os05g0530500

  O. sativa

  XP_015639849.1

  SNF1-related protein kinase catalytic subunit alpha KIN10

  505

Implicated in

Top note
AMPK, a central switch determining the AMP/ATP ratio, is dysregulated in several human diseases including diabetes and metabolic syndrome, cardiovascular diseases, neurodegenerative diseases and several different cancer types (Steinberg and Kemp, 2009). Both isoforms of AMPK: AMPK α1 and AMPK α2 may be involved in these diseases. AMPK was shown to negatively regulate the Warburg effect in genetically ablated AMPK- α1 cancer models in vivo (Faubert et al., 2013); therefore, AMPK can be classified as tumour suppressor although there is also evidence of negative regulation of AMPK by tumour suppressors or proto-oncogenes (Li et al., 2017; Yan et al., 2014).
Entity name
Huntingtons Disease
Note
Huntingtons disease (HD) is a neurodegenerative disease where the AMPKα1 isoform is known to be activated in the caudate nucleus and frontal cortex of humans. Activated AMPKα1 was reported to accumulate in the nuclei in these specific regions of the brain of HD patients. Brain atrophy, facilitated neuronal loss and increased aggregation of huntingtin ( HTT) protein was observed in a transgenic mouse model with Huntingtons disease, which had overactivated AMPKα1. Ameliorated cell death and down-regulation of BCL2 (by mutant Htt) was achieved by prevention of nuclear translocation or inactivation of AMPK- α1 (Ju et al., 2011).
Entity name
Prostate Cancer
Note
In prostate cancer, the androgen receptor ( AR) plays a critical role in the regulation of cell proliferation and death. There is evidence that AR related progression of prostate cancer correlates with activated AMPK levels. Androgen-mediated AMPK activity was reported to increase the levels of intracellular ATP and PPARGC1A (peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α))-mediated mitochondrial biogenesis. siRNA-mediated knockdown of AMPKα1, the predominant isoform correlated with poor prognosis in prostate cancer patients, in LNCaP and YCaP human prostate cancer cells reduced the levels of PGC-1α, which is overexpressed in clinical cancer samples (Tennakoon et al., 2015).
5- ATIC (Aminoimidazole-4-carboxamide ribonucleotide (AICAR)), is an AMPK agonist that enhances phosphorylation of AMPK- α1 at Thr-172 and its downstream target ACC at Ser-79. Prostate cancer cell lines infected with lentiviral shRNA against AMPK- α1 were shown to almost block AICAR-induced AMPK phosphorylation. AICAR-induced cytotoxicity in prostate cancer cells was slightly more potent than other AMPK activators such as A-769662 and Compound 13. It has been suggested that AICAR-induced cytotoxicity was not dependent of AMPK activation but might play a pro-survival role in prostate cancer cells (Guo et al., 2016).
Entity name
Colorectal Cancer
Note
The current literature suggests that activation of AMPK through natural compounds such as berberine, epigallocatechin gallate or quercetin can enhance apoptosis through the upregulation and phosphorylation of TP53 at Ser15, inhibition of COX-2 and mitigation of inflammation as well as delay in cell cycle progression (Sun and Xhu, 2017). AMPKα1 is expressed in almost all colorectal cancer cell lines; however, AMPKα2 expression is limited to some cell lines. Although siRNA-mediated AMPKα1 knock down has no effect on cell death, AMPKα2 depletion was shown to induce cell death in both HCT116 and SW480 cell lines. A competitive inhibitor of AMPK, 5-hydroxy-staurosporine, was identified by FUSION (Functional Signature Ontology), a method to screen natural compounds for the identification of AMPK inhibitors. Colorectal cancer cell lines were reported to be more sensitive to 5-hydroxy-staurosporine compared to non-transformed human colon epithelial cells (Das et al., 2018).
Another study suggests that Icaritin (a flavonoid with anti-tumorigenic activity) was reported to induce AMPK signaling in colorectal cancer (CRC) and it also activates autophagy. AMPK-α1 knockdown (shRNA or siRNA mediated) inhibited icaritin-activated autophagy but increased cell death in CRC both in vitro and in vivo (Zhou et al., 2017).
Entity name
Type 2 Diabetes
Note
AMPK is known to be dysregulated in patients with metabolic syndrome or type 2 Diabetes. Activation of AMPK either through the alteration of the AMP/ATP ratio of by pharmacological agonists can improve insulin sensitivity and metabolic health. In the primary metabolic tissues such as skeletal muscles, cardiac muscle, liver and adipose tissue, activation of AMPK was reported to stimulate glucose uptake, fatty acid oxidation, glucose transporter type (GLUT)4 translocation (in skeletal muscles), mitochondrial biogenesis, while inhibiting gluconeogenesis (in the liver) as well as protein, fatty acid, cholesterol and glycogen synthesis. AMPK is also known to inhibit insulin secretion from pancreatic β-cells and can signals to enhance food intake in the hypothalamus. All of these are beneficial for Type 2 diabetes (Coughlan et al., 2014). In an animal model of type 2 diabetes established by the Otsuka Long-Evans Tokushima Fatty (OLETF) rat, which had chronic and slowly progressive hyperglycemia and hyperlipidemia, overexpression of adenoviral-mediated AMPK-α1 showed a modest decrease in blood glucose level although glucose tolerance was not recovered completely. Moreover, plasma triglyceride level and hepatic triglyceride contents were also slightly decreased (Seo et al., 2009).
Entity name
Aging
Note
Dietary restriction (DR), a process of reduced food intake without inducing malnutrition, elicits a low-energy state in the organism, which in turn delays ageing in species ranging from yeast to primates through the activation of nutrient-sensing pathways such as AMPK (Burkewitz et al, 2014). For example, feeding C. elegans 2-deoxy-D glucose leading to the inhibition of glycolysis and glucose metabolism increased the lifespan of the worms in an aak-2 (catalytic subunit of AMPK in C. elegans) dependent manner (Schulz et al., 2007). In rat EDL (extensor digitorum longus) muscle, AMPK-α1 protein level was reported to be higher in older rats compared to younger rats. On the other hand, young rats showed higher expression of AMPK-α2 proteins than the older group. EDL cells treated with AICAR showed increased AMPK-α2 activity in both age groups, while AMPK-α1 activity was increased only in the young group. AMPK-α1 activity was not changed in the EDL muscles that were stimulated by high frequency electrical in the young group (Thompson et al., 2009).

Article Bibliography

Pubmed IDLast YearTitleAuthors
192456552009The regulation and function of mammalian AMPK-related kinases.Bright NJ et al
179501002007The mammalian target of rapamycin regulates lipid metabolism in primary cultures of rat hepatocytes.Brown NF et al
247263832014AMPK at the nexus of energetics and aging.Burkewitz K et al
196903282009mTORC1 phosphorylates the ULK1-mAtg13-FIP200 autophagy regulatory complex.Chan EY et al
250186452014AMPK activation: a therapeutic target for type 2 diabetes?Coughlan KA et al
98570771998Functional domains of the alpha1 catalytic subunit of the AMP-activated protein kinase.Crute BE et al
294914752018A Functional Signature Ontology (FUSION) screen detects an AMPK inhibitor with selective toxicity toward human colon tumor cells.Das B et al
232740862013AMPK is a negative regulator of the Warburg effect and suppresses tumor growth in vivo.Faubert B et al
119030592002Characterization of the role of the AMP-activated protein kinase in the stimulation of glucose transport in skeletal muscle cells.Fryer LG et al
271034402016AICAR induces AMPK-independent programmed necrosis in prostate cancer cells.Guo F et al
184399002008AMPK phosphorylation of raptor mediates a metabolic checkpoint.Gwinn DM et al
219377102011AMP-activated protein kinase: an energy sensor that regulates all aspects of cell function.Hardie DG et al
249606952014Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids.Hofbauer HF et al
121948242002Activation of AMP-activated protein kinase leads to the phosphorylation of elongation factor 2 and an inhibition of protein synthesis.Horman S et al
184661152008The TSC1-TSC2 complex: a molecular switchboard controlling cell growth.Huang J et al
158661712005AMP-activated protein kinase induces a p53-dependent metabolic checkpoint.Jones RG et al
217682912011Nuclear translocation of AMPK-alpha1 potentiates striatal neurodegeneration in Huntington's disease.Ju TC et al
227867462012Reduced expression of FASN through SREBP-1 down-regulation is responsible for hypoxic cell death in HepG2 cells.Jung SY et al
199481452009An emerging role of mTOR in lipid biosynthesis.Laplante M et al
227286512012AMPK promotes p53 acetylation via phosphorylation and inactivation of SIRT1 in liver cancer cells.Lee CW et al
174860972007Energy-dependent regulation of cell structure by AMP-activated protein kinase.Lee JH et al
206889142010AMPK-dependent repression of hepatic gluconeogenesis via disruption of CREB.CRTC2 complex by orphan nuclear receptor small heterodimer partner.Lee JM et al
285401632017Dissecting the role of AMP-activated protein kinase in human diseases.Li J et al
172377712007The energy sensing LKB1-AMPK pathway regulates p27(kip1) phosphorylation mediating the decision to enter autophagy or apoptosis.Liang J et al
149765522004LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1.Lizcano JM et al
110691052000Phosphorylation and activation of heart PFK-2 by AMPK has a role in the stimulation of glycolysis during ischaemia.Marsin AS et al
184812022007Role of the PI3-kinase/mTor pathway in the regulation of the stearoyl CoA desaturase (SCD1) gene expression by insulin in liver.Mauvoisin D et al
94355251997AICA riboside increases AMP-activated protein kinase, fatty acid oxidation, and glucose uptake in rat muscle.Merrill GF et al
218921422011The AMPK signalling pathway coordinates cell growth, autophagy and metabolism.Mihaylova MM et al
204955552010AMPK controls the speed of microtubule polymerization and directional cell migration through CLIP-170 phosphorylation.Nakano A et al
223335802012Subunit composition of AMPK trimers present in the cytokinetic apparatus: Implications for drug target identification.Pinter K et al
216285302011AMPK -> ULK1 -> autophagy.Roach PJ et al
179085572007Glucose restriction extends Caenorhabditis elegans life span by inducing mitochondrial respiration and increasing oxidative stress.Schulz TJ et al
200544912009Overexpression of AMPKalpha1 Ameliorates Fatty Liver in Hyperlipidemic Diabetic Rats.Seo E et al
85576601996Mammalian AMP-activated protein kinase subfamily.Stapleton D et al
195843202009AMPK in Health and Disease.Steinberg GR et al
288355702017AMP-activated protein kinase: a therapeutic target in intestinal diseases.Sun X et al
170855802006A pivotal role for endogenous TGF-beta-activated kinase-1 in the LKB1/AMP-activated protein kinase energy-sensor pathway.Xie M et al
241862072014Androgens regulate prostate cancer cell growth via an AMPK-PGC-1α-mediated metabolic switch.Tennakoon JB et al
192735782009AMP-activated protein kinase response to contractions and treatment with the AMPK activator AICAR in young adult and old skeletal muscle.Thomson DM et al
272266232016Calcium-Oxidant Signaling Network Regulates AMP-activated Protein Kinase (AMPK) Activation upon Matrix Deprivation.Sundararaman A et al
247624382014The tumor suppressor folliculin regulates AMPK-dependent metabolic transformation.Yan M et al
213830162011AMP-activated protein kinase (AMPK) activation and glycogen synthase kinase-3β (GSK-3β) inhibition induce Ca2+-independent deposition of tight junction components at the plasma membrane.Zhang L et al
281035822017AMPK-autophagy inhibition sensitizes icaritin-induced anti-colorectal cancer cell activity.Zhou C et al

Other Information

Locus ID:

NCBI: 5562
MIM: 602739
HGNC: 9376
Ensembl: ENSG00000132356

Variants:

dbSNP: 5562
ClinVar: 5562
TCGA: ENSG00000132356
COSMIC: PRKAA1

RNA/Proteins

Gene IDTranscript IDUniprot
ENSG00000132356ENST00000296800Q96E92
ENSG00000132356ENST00000354209Q13131
ENSG00000132356ENST00000397128Q13131

Expression (GTEx)

0
10
20
30
40
50
60
Adipose - Subcutaneous
Adipose - Visceral
Adrenal Gland
Artery - Aorta
Artery - Tibial
Bladder
Brain - Amygdala
Brain - Anterior cingulate cortex
Brain - Caudate
Brain - Cerebellar Hemisphere
Brain - Cerebellum
Brain - Cortex
Brain - Frontal Cortex
Brain - Hippocampus
Brain - Hypothalamus
Brain - Nucleus accumbens
Brain - Putamen
Brain - Spinal cord
Brain - Substantia nigra
Breast - Mammary Tissue
Cells - Cultured fibroblasts
Cells - EBV - transformed lymphocytes
Cervix - Ectocervix
Cervix - Endocervix
Colon - Sigmoid
Colon - Transverse
Esophagus - Gastroesophageal Junction
Esophagus - Mucosa
Esophagus - Muscularis
Fallopian Tube
Heart - Atrial Appendage
Heart - Left Ventricle
Kidney - Cortex
Kidney - Medulla
Liver
Lung
Minor Salivary Gland
Muscle - Skeletal
Nerve - Tibial
Ovary
Pancreas
Pituitary
Prostate
Skin - Not Sun Exposed
Skin - Sun Exposed
Small Intestine - Terminal Ileum
Spleen
Stomach
Testis
Thyroid
Uterus
Vagina
Whole Blood

Pathways

PathwaySourceExternal ID
Autophagy - animalKEGGko04140
mTOR signaling pathwayKEGGko04150
Tight junctionKEGGko04530
Circadian rhythmKEGGko04710
Insulin signaling pathwayKEGGko04910
Adipocytokine signaling pathwayKEGGko04920
Autophagy - animalKEGGhsa04140
mTOR signaling pathwayKEGGhsa04150
Tight junctionKEGGhsa04530
Circadian rhythmKEGGhsa04710
Insulin signaling pathwayKEGGhsa04910
Adipocytokine signaling pathwayKEGGhsa04920
Hypertrophic cardiomyopathy (HCM)KEGGko05410
Hypertrophic cardiomyopathy (HCM)KEGGhsa05410
PI3K-Akt signaling pathwayKEGGhsa04151
PI3K-Akt signaling pathwayKEGGko04151
Non-alcoholic fatty liver disease (NAFLD)KEGGhsa04932
Non-alcoholic fatty liver disease (NAFLD)KEGGko04932
FoxO signaling pathwayKEGGhsa04068
Oxytocin signaling pathwayKEGGhsa04921
Oxytocin signaling pathwayKEGGko04921
AMPK signaling pathwayKEGGhsa04152
AMPK signaling pathwayKEGGko04152
Glucagon signaling pathwayKEGGhsa04922
Glucagon signaling pathwayKEGGko04922
Signal TransductionREACTOMER-HSA-162582
Signaling by Insulin receptorREACTOMER-HSA-74752
Insulin receptor signalling cascadeREACTOMER-HSA-74751
IRS-mediated signallingREACTOMER-HSA-112399
PI3K CascadeREACTOMER-HSA-109704
PKB-mediated eventsREACTOMER-HSA-109703
mTOR signallingREACTOMER-HSA-165159
Energy dependent regulation of mTOR by LKB1-AMPKREACTOMER-HSA-380972
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)REACTOMER-HSA-2404192
IGF1R signaling cascadeREACTOMER-HSA-2428924
IRS-related events triggered by IGF1RREACTOMER-HSA-2428928
Gene ExpressionREACTOMER-HSA-74160
Generic Transcription PathwayREACTOMER-HSA-212436
Transcriptional Regulation by TP53REACTOMER-HSA-3700989
TP53 Regulates Metabolic GenesREACTOMER-HSA-5628897
Cellular responses to stressREACTOMER-HSA-2262752
MacroautophagyREACTOMER-HSA-1632852
Insulin resistanceKEGGhsa04931
Longevity regulating pathwayKEGGhsa04211
Longevity regulating pathway - multiple speciesKEGGko04213
Longevity regulating pathway - multiple speciesKEGGhsa04213
Regulation of TP53 ActivityREACTOMER-HSA-5633007
Regulation of TP53 Activity through PhosphorylationREACTOMER-HSA-6804756
Fluid shear stress and atherosclerosisKEGGko05418
Fluid shear stress and atherosclerosisKEGGhsa05418
Apelin signaling pathwayKEGGhsa04371

Protein levels (Protein atlas)

Not detected
Low
Medium
High
cerebral cortex
cerebellum
hippocampus
caudate
thyroid gland
parathyroid gland
adrenal gland
nasopharynx
bronchus
lung
oral mucosa
salivary gland
esophagus
stomach 1
stomach 2
duodenum
small intestine
colon
rectum
liver
gallbladder
pancreas
kidney
urinary bladder
testis
epididymis
seminal vesicle
prostate
vagina
ovary
fallopian tube
endometrium 1
endometrium 2
cervix, uterine
placenta
breast
heart muscle
smooth muscle
skeletal muscle
soft tissue 2
adipose tissue
skin 1
skin 2
appendix
spleen
lymph node
tonsil
bone marrow

PharmGKB

Entity IDNameTypeEvidenceAssociationPKPDPMIDs
PA134983031GPAMGenePathwayassociated22722338
PA142672073CRTC2GenePathwayassociated22722338
PA189HMGCRGenePathwayassociated22722338
PA24421ACACAGenePathwayassociated22722338
PA24422ACACBGenePathwayassociated22722338
PA30861MLYCDGenePathwayassociated22722338
PA335SREBF1GenePathwayassociated22722338
PA35879SLC2A4GenePathwayassociated22722338
PA36198STK11GenePathwayassociated22722338
PA37353MLXIPLGenePathwayassociated22722338
PA37935SIRT1GenePathwayassociated22722338
PA450395metforminChemicalPathwayassociated22722338
PA61ATMGenePathwayassociated22722338

References

Pubmed IDYearTitleCitations
377735742024Gene expression of protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), solute carrier family 2 member 1 (SLC2A1) and mechanistic target of rapamycin (MTOR) in metformin-treated type 2 diabetes patients with COVID-19: impact on inflammation markers.2
381664852024Nuclear DNA damage-triggered ATM-dependent AMPK activation regulates the mitochondrial radiation response.0
382116512024AMPKα1-mediated ZDHHC8 phosphorylation promotes the palmitoylation of SLC7A11 to facilitate ferroptosis resistance in glioblastoma.3
382290382024NUSAP1 promotes pancreatic ductal adenocarcinoma progression by drives the epithelial-mesenchymal transition and reduces AMPK phosphorylation.2
386425012024SLC7A11-ROS/αKG-AMPK axis regulates liver inflammation through mitophagy and impairs liver fibrosis and NASH progression.2
377735742024Gene expression of protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), solute carrier family 2 member 1 (SLC2A1) and mechanistic target of rapamycin (MTOR) in metformin-treated type 2 diabetes patients with COVID-19: impact on inflammation markers.2
381664852024Nuclear DNA damage-triggered ATM-dependent AMPK activation regulates the mitochondrial radiation response.0
382116512024AMPKα1-mediated ZDHHC8 phosphorylation promotes the palmitoylation of SLC7A11 to facilitate ferroptosis resistance in glioblastoma.3
382290382024NUSAP1 promotes pancreatic ductal adenocarcinoma progression by drives the epithelial-mesenchymal transition and reduces AMPK phosphorylation.2
386425012024SLC7A11-ROS/αKG-AMPK axis regulates liver inflammation through mitophagy and impairs liver fibrosis and NASH progression.2
356805152023Impacts of altered exercise volume, intensity, and duration on the activation of AMPK and CaMKII and increases in PGC-1α mRNA.4
364685392023AMP-activated protein kinase is a key regulator of obesity-associated factors.0
370809952023RBM4 dictates ESCC cell fate switch from cellular senescence to glutamine-addiction survival through inhibiting LKB1-AMPK-axis.6
372246502023Nesfatin-1, a novel energy-regulating peptide, alleviates pulmonary fibrosis by blocking TGF-β1/Smad pathway in an AMPKα-dependent manner.0
373651502023Sex and age differences in AMPK phosphorylation, mitochondrial homeostasis, and inflammation in hearts from inflammatory cardiomyopathy patients.7

Citation

Esin Gülce Seza ; Ismail Güderer ; Çagdas Ermis ; Sreeparna Banerjee

PRKAA1 (protein kinase AMP-activated catalytic subunit alpha 1)

Atlas Genet Cytogenet Oncol Haematol. 2018-07-01

Online version: http://atlasgeneticsoncology.org/gene/43428/prkaa1-(protein-kinase-amp-activated-catalytic-subunit-alpha-1)